摘要
番茄叶霉菌小种４ 是番茄Ｃｆ５ 品系的非亲和小种，接种Ｃｆ５ 植株第 ３ 叶后，经不同诱导间隔期以亲和小种５ 接种第３ 叶和第４ 叶，１５ ｄ 后检查叶霉病发病情况。试验表明，在诱导间隔期为３ ｄ 和５ ｄ 时，小种４ 诱导接种的第 ３ 叶和未经诱导接种的上位第 ４ 叶发病面积比不接种或接种小种５ 的对照显著降低，以５ ｄ 间隔期处理效果最好。上述２ 个叶位的发病分别比对照降低９０％ 和８５％ 。小种４ 接种第３ 叶后该叶位和上部未接种第４ 叶内水杨酸含量迅速增加，以接种后３ ｄ 含量最高，分别达４０２ μｇ／ｇ 鲜重和３２１μｇ／ｇ 鲜重，比对照分别高２ 倍和 １８ 倍。接种后５ ｄ 内始终保持较高水平。接种８ ｄ 后逐渐下降，但仍高于对照。水杨酸含量的增加早于抗性表现，因而可能在该系统的抗性诱导中起作用。
Cladosporium fulvum race 4 which is incompatible to Cf5 tomato plants was inoculated on the third leaves of Cf5 plants.The compatible race 5 were then challenge inoculated on the third and fourth leaves at different intervals and disease areas on these leaves were recorded 15 days after the challenge inoculation. Results showed that the disease areas on the third leaf inoculated with race 4 and on the uninoculated fourth leaf were significantly reduced when the intervals between initial inoculation and the challenge inoculation were 3 or 5 days.The best challenge interval was 5 days resulting in disease reduction on the third and fourth leaves by 90% and 85%,respectively.Salicylic acid(SA) was found to accumulate rapidly in the inoculated leaf 3 and the uninoculated leaf 4 with maximum of 4.02 μg/g FW and 3.21 μg/g FW in the leaves 3 days after the induction inoculation,which were 2 and 1.8 times higher than those of control,respectively.High SA levels persist for 5 days and started to decline 8 days after the inoculation,but they were still higher than those of control.The accumulation of SA preceded the presence of resistance indicated that SA may involved in the resistance induction in tomato plants.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第3期261-264,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金
浙江省自然科学基金
瑞典IFS资助
关键词
番茄
基因互作
系统诱导抗性
水杨酸
叶霉菌
tomato,gene interactions,systemic induced resistance,salicylic acid
