摘要
目的探讨安徽省主要传疟媒介中华按蚊拟除虫菊酯击倒抗性机制,并为进一步建立抗性检测分子方法提供基础。方法采用自行设计的引物扩增中华按蚊钠离子通道ⅡS4-S6节段基因序列,扩增产物双向测序,获得的基因序列与GenBank数据库进行比对。结果受试中华按蚊均扩增出约378 bp均一清晰条带。测序比对发现,在相对于冈比亚按蚊钠离子通道1 013和1 014位置发生突变:由TTG突变为TTT或TGT,即L/F或L/C突变。结论安徽省中华按蚊已发生针对拟除虫菊酯的击倒抗性突变。
Objective To study the mechanism for pyrethroid knockdown resistance of Anopheles sinensis,the main malaria vector in Anhui Province,and provide some evidence for developing a method of its molecular detection.Methods Primers were designed by the authors to amplify the voltage-gated sodium channel（VGSC） gene ⅡS4-S6.PCR products were sequenced in both directions,and homology searches were done over the Web using the program Blast.Results Fragments of 378 bp were amplified and resistance mutations in comparison to the Anopheles gambiae VGSC gene were found at the 1 013（T-G） and 1 014（G-T） positions,a result of L/F or L/C amino acid replacement.Conclusion The mechanism for mutations resulting in the knockdown resistance of A.sinensis in Anhui Province has been determined.
出处
《中国病原生物学杂志》
CSCD
2010年第12期922-924,934,共4页
Journal of Pathogen Biology
基金
国家自然科学基金青年项目(No.30901255)
安徽省优秀青年教师基金项目(No.2009SQRZ119)
卫生行业科研专项(No.200802021)
第五轮中国全球基金疟疾项目实施性研究专项基金(No.GF/M5/2010-04)
安徽省自然科学基金项目(No.10040606Q38)
关键词
中华按蚊
拟除虫菊酯
击倒抗性
Anopheles sinensis
pyrethroid
knockdown resistance
