摘要
目的:建立并评价2种复合型老年失眠证候大鼠模型。方法:分别采用腹腔注射D-半乳糖+夹尾刺激+睡眠剥夺,腹腔注射D-半乳糖+环磷酰胺及氢化可的松+睡眠剥夺制作肝郁、血亏阴虚型老年失眠证候大鼠模型,观测大鼠体征、脑部自由基水平、血浆6-keto-PGF1α、TXB2及血象含量。结果:与空白对照组、单因素模型组比较,2种模型组的自由基水平均显著增高(P<0.05,P<0.01);与其他组比较,老年肝郁失眠证候模型大鼠TXB2含量显著降低,6-keto-PGF1α含量及6-keto-PGF1α/TXB2比值均显著升高(P<0.01),而老年血亏阴虚失眠证候模型大鼠全血血象显著降低(P<0.01)。结论:老年肝郁及血亏阴虚失眠证候大鼠模型均具有临床证候病理特征,是可行、可靠的复合型动物模型。
Objective： To establish and evaluate the two kinds of aging rat pathogeny models of insomnia.Methods： The insomnia rats model with the pathogeny of aging Liver-qi stagnationwas induced by D-galactose neck back subcutaneously,the stimulation of the rats tail leading to Liver-qi stagnation,sleep deprivation using modified multiple platform method（MMPM）.The other insomnia rats model with the pathogeny of aging Blood-deficiency and Yin was induced by D-galactose neck back subcutaneously,intraperitoneal injection of cyclophosphamide and hydrocortisone,sleep deprivation using MMPM.Then observed the morphology,free radical in brain,and the contents of 6-keto-PGF1α,TXB2 in blood plasma.Whole blood hemogram.Results： compared with control group and single-factor model group,the two multiple factors model groups all showed the higher levels of free radical（P0.01）.Compared with the other groups,multiple factors model group with aging Liver-qi stagnation showed the higher levels of TXB2,while the lower levels of 6-keto-PGF1α and the rate of 6-keto-PGF1α / TXB2 significantly（P0.01）,the other multiple factors model group with blood-deficiency and Yin showed the lower levels of the whole blood hemogram significantly（P0.01）.Conclusion： The two kinds of aging rat pathogeny models of insomnia that have pathological features of clinical syndromes,were viable,reliable animal model of the compound.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第1期123-126,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金2010年面上项目(No.81072849)
武汉市科技攻关计划项目(No.200960323121)~~
关键词
大鼠模型
睡眠剥夺
老年
肝郁
血亏阴虚
Rats model
Sleep deprivation（SD）
Aging
Liver-qi stagnation
Blood-deficiency and yin
