摘要
研究肠安宁胶囊对大鼠溃疡性结肠炎(UC)的实验治疗作用。采用乙酸化学刺激法建立大鼠UC模型,造模成功后将大鼠随机分为正常组、模型组、西药阳性药物组(SASP)、中药阳性药物组(肠胃宁胶囊)以及肠安宁胶囊低、中、高剂量组。造模后各给药组治疗3周,正常组和模型组正常饲养。实验过程中观察大鼠的精神、活动等一般状态。实验结束后,取血测定炎症及免疫细胞、解剖观察并做结肠组织病理切片。结果表明,与模型组比较,在治疗3周后,除肠安宁胶囊低剂量组外,各给药组大鼠一般状态明显改善;肉眼观察和组织病理学切片表明肠安宁胶囊有效改善了UC症状;肠重比结果显示肠安宁胶囊治疗大鼠UC后大鼠结肠无异常增厚现象;肠安宁胶囊组白细胞总数和中性粒细胞数明显减少(P<0.05),T细胞亚群CD4+/CD8+比率增大(P<0.01),表明肠安宁胶囊能减轻炎症症状,增强免疫功能。实验结果表明肠安宁胶囊高剂量对实验性UC有明显的治疗作用。
To study the experimental treatment effect of Chang＇anning capsules,the rat model of ulcerative colitis（UC） was established by using acetic acid.After the preparation of the ulcerative colitis rat model,the rats were randomly divided into six groups,including normal control group,model control group,western medicine positive control group（SASP）,TCM positive control group,small,medium and large dose group of Chang＇anning capsules.Each group was treated for three weeks.The normal and model control groups were fed using the standard method.During the study,the general activities and appearance of the rats were observed.After the experiment,blood withdrawal,dissection and pathological section were conducted while examination of the colon mucosa and detection of both the inflammation and immune cells were carried out.In comparison to the model group,the rats＇ general states of all treatment groups were better than that of model group in three weeks treatment.Macroscopic observation and histopathological section indicated the marked obvious effect of Chang＇anning capsules on UC rats,and there existed no abnormal thickening in the rats＇ colon after the treatment of Chang＇anning capsules.Leucocyte and neutrophile granulocytes were significantly less than those of the model group（P0.05）,but the T cell subgroup CD4＋ /CD8＋ was larger than that of the model group（P0.01）,which could indicate that Chang＇anning capsules could decrease inflammation and increase the immune function.It was concluded that Chang＇anning capsules（higher dose）has significant treatment effect on the experimental ulcerative colitis of rats.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2010年第6期563-567,共5页
Journal of China Pharmaceutical University
关键词
肠安宁胶囊
溃疡性结肠炎
乙酸
组织病理学
Chang＇anning capsules
ulcerative colitis
acetic acid
histopathology
