摘要
目的观察牛肾上腺嗜铬细胞异种移植于帕金森病（ＰＤ）样大鼠脑内的效果及移植物的存活情况。方法将用酶消化和机械分离及纯化的牛肾上腺嗜铬细胞移植于ＰＤ样大鼠的脑纹状体内（损毁同侧），以阿朴吗啡诱发的异常旋转行为为观察指标，比较细胞移植组、生理盐水组和非移植组间及移植前、后旋转行为的变化。结果细胞移植组的ＰＤ样大鼠移植后的旋转行为较移植前明显改善（Ｐ＜０．０１），而生理盐水组和非移植组的改善不明显；移植后细胞移植组与生理盐水组、非移植组比较，旋转行为的改善较后两组明显改善（Ｐ＜０．０１），此结果至少可保持半年。细胞移植组的大鼠于移植半年时行移植部组织形态学检查（ＨＥ、ＴＨ染色），证明牛嗜铬细胞在大鼠脑内存活。结论牛肾上腺嗜铬细胞移植于ＰＤ样大鼠脑内，不仅可以存活。
Objective To assess the effects
and the graft survival of xenotransplantation of bovine chromaffin cells into brain of
hemiparkinsonian rats. Methods The enzymedigested, mechanically isolated and purified
bovine chromaffin cells were implanted into the striatal (at the same side of the destroyed) of
hemiparkinsonian rats. Two weeks after xenograft, the apomarphineinduced rotational
asymmetry served as an indicator. The rotational behavioral changes among the groups of the
cell graft, saline and nograft and before and after transplantation were compared. Results In the
cell graft group, the rotational behaviors of the hemiparkinsonian rats after transplantation were
obviously improved as compared with those before transplantation (P<0.01),no significant
improvement was found in other two groups.The improvement of the rotational asymmetry in
the cell graft group after transplantation was better than in the saline group and nograft group
(P<0.01).The morphological examination (HE and TH staining) of the striatal sections in cell
graft group 24 weeks after transplantation indicated the survival of the bovine chromaffin cells
in the rat brain. Conclusion The transplantation of the bovine chromaffin cells in the brain of
hemiparkinsonian rats could correct the abnormal rotational behaviors and the xenografts could
survive.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第3期154-156,共3页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金
国家九五攻关项目基金
中国人民解放军医药卫生科研基金
关键词
震颤性麻痹
嗜铬细胞移植
行为学
组织学
Parkinson diseaseAdrenal medullaTransplantation,
heterologousCells/transplantationRats
