摘要
目的观察微囊化牛嗜铬细胞（ＢＣＣ）大鼠脑内移植的效果及微囊的作用。方法将微囊化或非微囊化ＢＣＣ和空微囊分别移植于帕金森病（ＰＤ）样大鼠脑纹状体内，观察术后阿朴吗啡诱发大鼠异常旋转行为的变化；用蔗糖磷钾酸乙醛酸（ＳＰＧ）荧光染色和ＨＥ染色观察脑组织中植入的ＢＣＣ及微囊的状态。结果空囊组ＰＤ样大鼠异常旋转行为改变不明显（ｎ＝６，Ｐ＞０．０５）；非微囊组１６只ＰＤ样大鼠中９只大鼠移植后１周旋转圈数下降到移植前的４４．０％～６０．９％（Ｐ＜０．０１），移植６个月时仍有ＢＣＣ在部分受体脑内存活，移植区有较明显的炎性反应，另有７只ＰＤ样大鼠移植后异常旋转行为无明显改善（Ｐ＞０．０５），其脑内未见存活的ＢＣＣ，移植区有较明显的炎性反应；微囊组１６只ＰＤ样大鼠移植后旋转圈数下降到移植前的１７．６％～３５．６％（Ｐ＜０．０１），移植后１０个月时大鼠脑内仍存在大量存活的微囊化ＢＣＣ，无明显的炎性反应。微囊化ＢＣＣ移植改善ＰＤ样大鼠异常旋转行为的效果明显优于非微囊者（Ｐ＜０．０１）。结论ＢＣＣ脑内移植可改善ＰＤ样大鼠的异常旋转行为；大鼠旋转行为的改善与ＢＣＣ在脑内的存活状态有关；海藻酸钠及多聚赖氨酸制作的微囊可降低异种ＢＣＣ?
Objective To observe the immune separation effects of micro capsules from alginate polylysine alginate(APA)in the rat cerebral transplantation of bovine chromaffin cell(BCC) grafts.Methods Microencapsulated BCC, non microencapsulated BCC or empty microencapsulated BCC were grafted into the striatum of the hemiparkinsonian disease(PD) like rats respectively. The abnormal rotational behavior induced by apomorphine was observed in the rats after operation. At the end of experiments the rats were killed and transcardially perfused with 300 ml normal saline. The brains were removed and frozen sections were cut in coronal plane on a freezing microtome. The BCC grafts and micro capsules in the brains were observed under a fluorescent microscope after SPG staining and HE staining. Results The changes in abnormal behavior of PD like rats in empty microencapsulated graft group were not significant in 10 months( P > 0.05 ). Among the 16 PD like rats in non microencapsulated graft group, 9 rats had an improvement of abnormal rotational behavior one week after transplantation, with the rotations being decreased to 44.0% ～ 60.9% of pregrafting in non microencapsulated BCC graft group ( P < 0.01 ). At the 10th month of postgrafting there were a large number of survival BCC in the host striatum with only minimum host reaction in microencapsulated BCC graft group. On the 6th month of postgrafting there was some survival BCC in the host corpus striatum accompanied by inflammatory response in non microencapsulated BCC graft group. There was a significant difference in apomorphine induced rotational behavior between microencapsulated BCC graft group and non microencapsulated BCC graft group ( P < 0.01 ). Conclusion Transplantation of BCC into brain can improve the abnormal rotational behavior in PD like rats, which was correlated with the survival of BCC in the host brain. The micro capsules from APA can decrease the host immune response to the heterogenic BCC grafts.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第1期10-12,共3页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金
国家计委"九五"重点科技攻关项目
军队"九五"医药卫生科研基金
关键词
震颤性麻痹
脑内移植
BCC
微囊化
牛嗜铬细胞
Parkinson disease
Adrenal medulla
Transplantation
heterologous
Biocompatible materials
Rats
