摘要
目的:研究细丝蛋白A(FLNa)在结直肠腺癌组织中的表达情况,探讨FLNa在结直肠腺癌发生发展中的作用。方法:选择2009年1月～9月河北医科大学第四医院外二科60例患者手术切除的结直肠腺癌组织和正常结直肠组织。癌组织取自肿瘤中心处,正常组织取自距肿瘤边缘至少100cm处。采用RT-PCR法和Western blot法检测60例结直肠腺癌患者癌组织和结直肠正常组织中FLNa的mRNA和蛋白质的表达情况。同时采用免疫组化法检测FLNa在结直肠癌组织和结直肠正常组织中的分布,并分析组间的FLNa阳性率表达差异。所有病例均经病理诊断确诊,均无其他部位原发肿瘤,术前无化疗、放疗和免疫治疗史。结果:结直肠癌组织中FLNa mRNA的表达水平(0.22±0.02)较结直肠正常组织低(0.94±0.04)(P=0.018);Western blot法检测显示:FLNa蛋白在结直肠癌组织中的表达水平(0.34±0.03)也较结直肠正常组织低(0.85±0.02)(P=0.028);Pearson相关分析显示:FLNamRNA和蛋白水平呈正相关(r=0.654,P<0.05)。FLNa免疫组化阳性染色产物位于细胞质。FLNa在结直肠癌组织组和结直肠正常组织组的阳性表达率分别为50.00%、91.67%,癌组织组明显低于正常组织组(x^2=50.037,P=0.000)。结论:FLNa的特异性低表达与结直肠腺癌的发生、发展具有高度相关性,有望成为新的肿瘤标志物,为临床诊治提供的新靶量。
Objective： To study the expression of filamin-a （FLNa） in human colorectal adenocarcinoma, and determine the role of FLNa in the pathogenesis of colorectal adenocarcinoma. Methods： The samples of colorectal adenocarcinoma and normal colorectal tissues were collected from 60 patients who underwent colorectal resection. Colorectal adenocarcinoma tissues were obtained from the central part of the tumor, while normal colorectal tissues were collected from the site which was at least 10 cm away from the edge of the lesion. Reverse transcription polymerase chain reaction （RT-PCR） and Western blot method were used to detect the expressions of FLNa mRNA and protein in the 60 specimens with colorectal adenocarcinoma and normal colorectal tissues. At the same time, immunohistochemistry was conducted to detect the distribution of FLNa protein in colorectal adenocarcinoma and normal colorectal tissues, and to analyze the differences between two groups. All cases were confirmed by pathological diagnosis, and pdmary tumors were not found at other sites. No medical records of preoperative radiotherapy, chemotherapy and immunotherapy were found in the groups. Results： The expression of FLNa mRNA was lower in colorectal adenocarcinoma than in normal colorectal tissues （P=0.018, 〈0.05）. Western blot analysis revealed that the expression of FLNa protein was also lower in colorecta adenocarcinoma than in the normal colorectal tissues （P=0.028 〈0.05）. Pearson correlation analysis showed that the mRNA level was in positive correlation with the protein level （r=0.645, P〈0.05）. The positively stained particles were distributed in the cytoplasm. Of the 60 colorectal adenocarcinoma samples, 30 （50%） cases were positive for FLNa expression. And of the 60 normal colorectal samples, 55 （91.67%） cases were positive for FLNa expression. The positive rate of FLNa expression was lower in colorectal adenocarcinoma samples than in the normal colorectal samples （x2=50.037, P=0.000〈0.05）. Immunohistochemical analysis showed the same results as RT-PCR and Western blot. Conclusion： The specific low expression of the FLNa protein is in a high correlation with the pathogenesis of colorectal adenocarcinoma. FLNa is expected to be a new tumor marker and the new target for clinical therapy.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2010年第23期1342-1345,共4页
Chinese Journal of Clinical Oncology
基金
河北省卫生厅医学科学研究重点课题计划(编号:20090010)
河北省普通高校强势特色学科建设基金资助(编号:冀高教[2005]52号)~~
