摘要
目的:观察钙敏感受体(CaSR)在缺氧诱导的大鼠肺动脉平滑肌细胞增殖中的作用。方法:Ⅱ型胶原酶消化法提取、培养大鼠肺动脉平滑肌细胞。应用Western blotting技术及免疫荧光染色分析CaSR蛋白在肺动脉平滑肌的表达;采用激光共聚焦扫描技术检测不同处理因素对细胞内钙离子浓度([Ca2+]i)的影响,应用MTT法分析不同处理因素对细胞存活率的影响。结果:大鼠肺动脉平滑肌细胞有CaSR蛋白表达。缺氧能够增加CaSR和增殖细胞核抗原(PCNA)的表达、细胞存活率及[Ca2+]i(与对照组比较,P<0.05)。GdCl3(CaSR激动剂)能够增强缺氧的上述作用,NPS2390(CaSR抑制剂)则能够减弱缺氧的作用。结论:缺氧诱导的CaSR表达增加参与了缺氧性肺动脉平滑肌细胞增殖。
AIM：To explore the role of calcium-sensing receptor（CaSR） in rat pulmonary artery smooth muscle cells（PASMCs） and its effect on hypoxia-induced proliferation of PASMCs.METHODS： The expression and distribution of CaSRs were detected by Western blotting and immunofluorescence observation.The intracellular concentration of free calcium（i） was determined by confocal laser scanning microscopy.The cell viability was analyzed by MTT assay.The expression of PCNA and CaSRs was determined by Western blotting.RESULTS： CaSR protein was expressed in rat PASMCs.Hypoxia significantly increased the expression of CaSR and PCNA,i and the cell viability.GdCl3（an agonist of CaSR） or NPS2390（an antagonist of CaSR） amplified or weakened the effect of hypoxia,respectively.CONCLUSION： CaSR is expressed in rat PASMCs.The activation of CaSR is involved in the proliferation of PASMCs induced by hypoxia.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第12期2433-2437,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30871012
No.30700288
No.80070123)
教育部博士点基金资助项目(No.20070226012)
黑龙江省研究生创新基金资助项目(No.YJSCX2009-223HLJ)
关键词
受体
钙敏感
肺动脉平滑肌细胞
缺氧
细胞增殖
Receptors
calcium-sensing
Pulmonary arterial smooth muscle cell
Hypoxia
Cell proliferation
