摘要
目的:从经穴效应量的差异探索不同部位、不同组织结构与经穴特异性的关系。方法:成年SD大鼠,选择不同经脉、不同部位组织结构相似的经穴("大陵"与"解溪""郄门"与"后三里""曲泽"与"犊鼻")及腹部非穴,电针刺激右侧单穴以及"郄门"和"后三里"的单一组织(皮肤、肌肉、骨膜,3个系列试验,每组12例),观察其对心得安所致心动过缓大鼠心率、平均动脉压(MAP)回升率的调节效应。结果:①模型组给心得安后,心率和MAP一直保持低水平。"郄门""曲泽""后三里""解溪"组的心率、MAP回升率分别是非穴组的7、8、6、6倍和4、4、3、3倍,差异有统计学意义(P<0.05,P<0.01);而"大陵""犊鼻"组与非穴组的心率、MAP回升率接近(P>0.05)。②同一经脉不同部位经穴的效应量呈现循经脉循行方向增强的趋势,即:"曲泽">"郄门">"大陵","解溪"与"后三里">"犊鼻"。③"郄门"与"后三里"同一穴位常规电针与不同组织电针效应量存在差异,基本表现为皮肤>肌肉≈常规电针>骨膜。④不同穴位相同组织效应量的差异部分有统计学意义。表现为电针"郄门"与"后三里"二穴皮肤,其在调节心率方面"郄门"优于"后三里"(P<0.01),电针二穴肌肉在调节MAP方面"郄门"优于"后三里"(P<0.01),电针二穴骨膜的效应量相当。⑤组织结构相似穴位的效应量差异具有统计学意义,"大陵"与"犊鼻"效应相似,"曲泽"与"解溪"效应相似,"后三里"和"郄门"效应相似,而"解溪"在心率效应量方面比"大陵"高出4倍、在MAP回升率方面高出3倍,"曲泽"在心率效应量方面是"犊鼻"的6倍、在MAP回升率方面是"犊鼻"的2.7倍。结论:不同经脉组织结构相似经穴的效应量存在特异性,同一组织在不同部位其效应量亦有特异性;当机体组织成为调节机体功能的途径时,必须重新认识不同部位组织功能的差异;不同部位组织与机体联系途径的差异及功能特化可能是构成其对刺激特异性反应的物质基础。
Objective To explore the relationship between the effect of electroacupuncture （EA） and EA of different layer tissues of the acupoint area and different acupoints in upregulating mean arterial pressure （MAP） and heart rate （HR） in hypotension plus bradycardia rats. Methods A total of 200 SD rats were used in the present study. Bradycardia plus hypotension model was established by intravenous injection of 0.4% propranolol （0.4 mg/100 g, maintaining dosage 0. 025 mg/100 g per minute）. EA （2 Hz /15 Hz, 5 mA） was applied to 1） right ＂Daling＂ （PC7） and ＂Jiexi＂ （ST 41）, ＂Ximen＂ （PC 4） and ＂Housanli＂ （ST 36）, ＂Quze＂ （PC 3） and ＂Dubi＂ （ST 35） which have a similar tissue structure, and are located in the upper and lower limbs and different meridians, and non-acupoint [3 mm left-superior to the ＂Tianshu＂ （ST 25）], 2） skin, muscle layer and periosteum part of ＂Ximen＂ （PC4）, 3） skin, muscle layer and periosteum of ＂Housanli＂ （ST 36） for 15 min. The HR and MAP were recorded by using a multi-channel physiological signal sampling-processing system. Results 1） In comparison with the model group, the percentages of the increased HR and MAP in the ＂Ximen＂ （PC 4）, ＂Quze＂ （PC 3）, ＂Housanli＂ （ST 36） and ＂Jiexi＂ （ST 41 ） groups, PC 4-skin, PC 4-muscle, PC 4-periosteum, ST 36-skin, ST 36-muscle and ST 36 periosteum groups, and the increased HR in the ＂Dubi＂ （ST 35） group were upregulated significantly （P〈0.05, P〈0.01）. The percentages of the increased HR and MAP were significantly higher in the ＂Quze＂ （PC 3） and ＂Ximen＂ （PC 4） groups than in the ＂Daling＂ （PC 7） group （P〈0.01）, and the increased HR evidently higher in the ＂Housanli＂ （ST 36） and ＂Jiexi＂ （ST 41） groups than in the ＂Dubi＂ （ST 35） group （P〈0.01）, suggesting different effects of EA stimulation of different acupoints in the same one meridian. No significant differences were found among the ＂Ximen＂ （PC 4）, ＂Quze＂ （PC 3）, ＂Housanli＂ （ST36） and ＂Jiexi＂ （ST41） groups, and between the ＂Daling＂ （PC 7） and model groups, and between the non-acupoint and model groups in the rising rates of both HR and MAP （P〉0.05）. 2） Regarding the effects of EA of different tissue layers in ＂Ximen＂ （PC 4） and ＂Housanli＂ （ST 36） areas, the rising rates of HR were markedly higher in the PC 4-skin group than in the ＂Ximen＂ （PC 4）, PC 4-muscle and PC 4-periosteum groups （P〈0.01）, and considerably higher in the ＂Housanli＂ （ST 36）, ST 36-skin, and ST 36-muscle groups than in ST 36-periosteum group （P〈0.05）. The rising rates of MAP were significantly higher in the PC 4-skin and PO 4-muscle groups than in the ＂Ximen＂ （PC 4） and PC 4-periosteum groups （P〈0.01 ）, and considerably higher in the ST 36-skin group than in the ＂Housanli＂ （ST 36）, ST 36-muscle and ST 36-periosteum groups （P^0.01）, suggesting different effects of EA stimulation of different tissue layers in the same one acupoint. No significant differences were found between the ＂Ximen＂ （PC 4） and PC 4-muscle groups, among the ＂Housanli＂ （ST 36）, ST 36-skin and ST 36-muscle groups in the rising rates of HR, between the ＂Ximen＂ （PC 4） and PO 4-perio- steum groups, and among the ＂Housanli＂ （ST 36）, ST 36-muscle and ST 36-periosteum groups in the rising rates of MAP （P〈0.05）. 3） The effect of the PC 4-skin group was significantly superior to that of the ST 36-skin group in increasing HR （P〈0.01）, and the effect of the PC 4-muscle group was obviously stronger than that of the ST 36-muscle group in raising MAP （P〈0.01）, suggesting different therapeutic effects of EA stimulation of the similar tissue in different meridian-acupoints. The effects of the ＂Ximen＂（PC 4） and ＂Housanli＂ （ST 36） groups in raising both HR and MAP, and that of the PC 4-muscle and ST 36- muscle groups in upregulaing HR, and that of the PO 4-periosteum and ST 36-periosteum groups in raising MAP were comparable （P〉0.05）. Conclusion EA of different acupoints of the same one meridian, the similar structure of different meridian acupoints and different tissue layers of the same one acupoint have their own relatively specific effects in upregulating HR and MAP in hypotension plus bradycardia rats.
出处
《针刺研究》
CAS
CSCD
北大核心
2010年第6期422-428,共7页
Acupuncture Research
基金
国家重点基础研究发展计划("973")资助(2006CB504506)
国家自然科学基金资助(30873307)
2006年度江苏省青蓝工程优秀中青年学术带头人培养项目资助
关键词
经穴
特异性
穴位结构
穴位位置
Acupoint
Specifity of efficacy
Structure of acupoint
Location of acupoint
