摘要
采用双氧水降解的方法获得了低分子量的琼脂糖,并用粘度法测定了降解琼脂糖的分子量,利用红外光谱测试了降解琼脂糖结构,采用散射光浊度仪对琼脂糖溶液的成凝胶过程进行了探讨,并通过动物试验测试了其降解特性。通过控制降解反应时间可以得到不同分子量的降解琼脂糖,降解15h后琼脂糖的粘均分子量稳定在1.47万。浊度分析结果表明,随着分子量的减小,琼脂糖溶液的成凝胶速度及凝胶的强度均明显下降。红外光谱分析表明,在降解过程中少量的醛基可通过硼氢化钠还原。体内降解试验表明,琼脂糖通过降低分子量可提高降解速度。小分子量的琼脂糖降低了其凝胶温度,并延长了凝胶时间,提高了体内降解速度,可望用于皮肤再生敷料等生物医学领域。
Low molecular weight agarose is obtained through the degradation of agarose with hydrogen peroxide. Its chemical structure is determined by IR spectroscopy. The molecular weights are measured by viseosimetry. The formation of agarose gel is monitored by scattered light turhidimeter. The degraded agarose are examined with acute toxicity test and subcutaneous implanting test. Various agaroses with different molecular weight are obtained by controlling the degradation time. Titration analysis proved that epoxy group can be coupled into agarose effectively. The molecular weight of agarose is kept at 14,700 after 15h degradation. With decrease of agarose molecule, agarose solution has lower gelling rate and lower gel strength. The degraded agarose has few aldhydrate groups and is reduced with NaBH4. The degraded agarose lost faster than the original one in subcutaneous implantation. The agarose with small molecular weight is obtained with hydrogen peroxide, and has low gel temperature and extends gel time and raises degradation rate. The modified agarose has promise in medical application.
基金
863计划(2007AA09Z436)
广东省教育厅生物材料重点实验室开放基金
211工程项目
关键词
降解
琼脂糖
医学应用
degradation, agarose, medical application
