摘要
目的:研究龟鹿二仙胶颗粒对自然衰老小鼠学习记忆能力的影响。方法:将自然老化的14月龄小鼠随机分成模型对照组、给药防治组及阳性对照组。药物加入饮水中自然饮入,连续给药3个月,检测前另选2月龄小鼠作为青年对照组,各组于末次给药的前4 d用跳台法检测错误反应的潜伏期和逃避反应的潜伏期,于末次给药的前2 d以避暗法检测错误反应的潜伏期和5 min内错误反应的次数。检测后采血分离血清、取脑进行冰浴匀浆,检测血清和脑匀浆的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)的含量。结果:与青年对照组相比老龄模型对照组小鼠错误反应的潜伏期显著缩短,逃避反应的潜伏期显著延长,错误反应次数显著增加,SOD,GSH-Px活力显著降低、MDA含量显著增加。与老龄模型对照组相比,各给药防治组老龄小鼠错误反应的潜伏期显著延长,逃避反应的潜伏期显著缩短,错误反应的次数显著减少,SOD,GSH-Px活力显著提高,MDA含量显著降低。结论:龟鹿二仙胶颗粒对自然衰老小鼠具有增强抗氧化功能,提高学习记忆能力的作用。
Objective：To study the influence of turtles and deer antlers granula on learning and memory in the natural aging mice. Method： The natural aging of 14 months mice were randomly divided into model control group, administration control group and the positive control group. Drugs was prepared in drinking water and administrated to the rats for consecutive 3 months. In addition, 2 month-old mice were used as the young control group. Wrong and escape response latencies with step-down test for each group was investigated 4 days before the last administration. Two days before the last administration the error response latency and error response times within 5 rain was detected by avoiding dark method. After the treatment of drugs, blood serum and brain homogenate were separated and prepared, superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） activity and malondialdehyde （ MDA ） content was detected. Result： Compared with young control group error response latency of aged model group mice was significantly shorter, escape response latency was significantly longer, the error reaction times increased significantly, SOD, GSH-Px activity significantly decreased, MDA content increased significantly. Compared with the aging model group, error response latency of the administration control group aged mice was significantly prolonged, escape response latency was significantly shortened, error response rate was significantly decreased, SOD, GSH-Px activities were significantly increased, MDA content was significantly lower.Conclusion：The turtle and deer antlers granula can enhance antioxidant and improve learning and memory for the natural aging mice.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期142-145,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
龟鹿二仙胶颗粒
自然衰老
学习记忆
抗氧化
turtle and deer antlers granula
natural aging
learning and memory
antioxidant
