摘要
以胰大豆蛋白胨培养基(TSB)为基础培养基,采用单因素和正交试验方法优化提高鳗源迟钝爱德华氏菌ETY细胞生物量的培养基与培养条件。通过试验,获得迟钝爱德华氏菌ETY的优化培养基配方为1 000mL水加入葡萄糖3.0g、胰蛋白胨10.0g、鱼粉蛋白胨10.0g、磷酸氢二钾2.5g、氯化钠5.0g;培养条件优化结果为接种量8%、温度25℃、pH 7.5、转速200r.min-1。迟钝爱德华氏菌ETY在此条件下培养20h,细胞生物量达1.575×1010 cfu.mL-1,较优化前培养的细胞生物量5.200×109 cfu.mL-1提高3倍。每吨液体培养基的成本较优化前下降41.36%。
Using tryptone soya broth medium,the culture conditions of Edwardsiella tarda（ETY） were optimized by a single factor and an orthogonal test to enhance the cell concentration.The results showed that the optimized medium contained 3.0 g of glucose,10.0 g of tryptone,10.0 g of peptone,2.50 g of K2HPO4 and 5.0 g of NaCl per liter.And,the most favorable fermentation conditions were at 25℃,with an initial pH of 7.5,on a stirring speed of 200 r·min-1 and with the inoculating ratio of 8%.Compared with the control,the cell concentration of ETY could be improved from 5.200×109cfu·mL-1 to 1.575×1010cfu·mL-1,i.e.,3 times increase.At the same time,the cost of liquid medium was reduced by 41.36% compared to the conventional formulation.
出处
《福建农业学报》
CAS
2010年第5期541-547,共7页
Fujian Journal of Agricultural Sciences
基金
农业部公益项目(nyhyzx07-043-15)
国家863项目(2008AA92501)
福建省科技计划项目(2009N2003)
