摘要
为寻找低毒高效的新型杀藻剂,研究了β-环糊精包合(β-CD)的叶绿酸铜和β-CD叶绿酸铜铵盐对蓝藻集胞藻(Synechocystissp.PCC6803)的抑杀作用。结果表明,β-CD叶绿酸铜和β-CD叶绿酸铜铵盐对集胞藻的抑杀作用显著高于CuSO4,2种包合物在c(Cu2+)分别为2.5和1.5μmol.L-1时可完全抑制藻生长。β-CD叶绿酸铜和β-CD叶绿酸铜铵盐使集胞藻细胞叶绿素a荧光强度下降,硝酸还原酶活性降低,诱导碱性磷酸酶活性、抗氧化酶系统活性以及膜透性升高(P<0.05)。认为β-CD叶绿酸铜和β-CD叶绿酸铜铵盐与无机Cu2+对集胞藻的毒害机理相似。
To search for a new type of algicide,low in toxicity but high in effect,inhibiting effects of β-cycloamylose-chathrated copper（β-CD） chlorophyllin and β-CD ammonium copper chlorophyllin on Synechocystis sp.PCC6803 were investigated.Results show that β-CD copper chlorophyllin and β-CD ammonium copper chlorophyllin were more effective in inhibiting Synechocystis than CuSO4,completely inhibiting growth of the alga when their Cu^2＋ concentration was 2.5 and 1.5 μmol·L^-1,separately.They could decrease the intensity of chlorophyll fluorescence and the activity of nitrate reductase of the alga,but induce the activities of its phosphatase and anti-oxidation system and improve its membrane permeability（P〈0.05）.It is presumed that the toxic mechanism of the β-CD copper chlorophyllin and β-CD ammonium copper chlorophyllin inhibiting Synechocystis was similar to that of inorganic copper ion.
出处
《生态与农村环境学报》
CAS
CSSCI
CSCD
北大核心
2010年第6期568-573,共6页
Journal of Ecology and Rural Environment
基金
江苏省科学技术厅社会发展项目(BS2007065)
关键词
叶绿酸
铜
集胞藻
杀藻剂
Β-环糊精
chlorophyllin
copper
Synechocystis sp.PCC6803
algicide
β-cycloamylose
