摘要
用不同质量分数铅胁迫的垂柳(Salix babylonica)叶片饲喂柳蓝叶甲(Plagiodera versicolora),并采用分光光度法测定其2、3龄和成虫体内SOD、CAT和POD活性以及自由基含量。结果表明:随着铅胁迫质量分数的增大,各龄期柳蓝叶甲体内3种抗氧化酶活性和自由基含量均表现出先上升后下降的趋势,即低质量分数的铅促使其活性升高,高质量分数的铅使其活性下降,且与对照差异均达到极显著水平(p<0.01)。2和3龄幼虫体内POD活性变化平缓,且明显低于成虫,表明柳蓝叶甲成虫抵抗铅胁迫的能力比幼虫强。
Activities of three protective enzymes （SOD,CAT and POD） and content of free radical were measured in larva and adult of Plagiodera versicolora fed with the leaves of Salix babylonica under different Pb stresses by spectrophotometric determination. Results show that three antioxidant enzyme activities and free radical content increase first and then decrease with increasing concentrations of Pb. That is to say,low concentrations of Pb increased the activities of SOD,CAT and POD,while high concentrations of Pb decreased enzyme activities. There was a very significant difference between the control and all the treatments （p0.01）. POD activity of 2nd and 3rd larvae changed little and was obviously lower than that of adult （p0.01）,indicating that the adult has stronger resistant ability to Pb stress than the larvae.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2010年第5期88-89,109,共3页
Journal of Northeast Forestry University
基金
河北省林业科学技术研究项目(0803015)资助
关键词
垂柳
柳蓝叶甲
铅胁迫
保护酶
自由基含量
Salix babylonica
Plagiodera versicolora
Pb stress
Protective enzymes
Free radical content
