摘要
目的 研究增殖和凋亡在放射治疗宫颈癌中的预后价值。方法 回顾性分析了４０ 例放疗未控和根据临床病理因素与之配对的４０ 例放疗治愈的ⅡＢ～ⅢＢ 期宫颈鳞癌患者的细胞增殖和凋亡情况。以免疫组化ＡＢＣ法检测增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ） ，凋亡细胞用末端转移酶（ＴｄＴ） 介导的脱氧核苷酸切口及末端标记法（ＴｄＴｍｅｄｉａｔｅｄ ｂｉｏｔｉｎｄＵＴＰｎｉｃｋ ｅｎｄ ｌａｂｅｌｉｎｇ，ＴＵＮＥＬ）进行检测。结果 放疗治愈组的ＰＣＮＡ为５．３８ ±３．２１ ，未控组为７ ．５０ ±３．１６（ Ｐ＜ ０ ．０１） ；治愈组每１０ 个高倍视野下的有丝分裂细胞数为６．３３±５．１５，未控组为１１ ．３３ ±７．５０（ Ｐ＜ ０ ．００１） ；治愈组每１０ 个高倍视野下的凋亡细胞数为２０．９０±８．１９，未控组为２７ ．１３±１７ ．２８（ Ｐ＜ ０．０５）；凋亡细胞数与分裂细胞数之比值（Ａ／Ｍ） 治愈组为４ ．９７ ±３．３１，未控组为３．３６±２．８３（ Ｐ＜ ０ ．０５） 。结论 ＰＣＮＡ、凋亡细胞数以及Ａ／Ｍ
Objective To study the prognostic value of cell proliferation and apoptosis in cervical cancer patients treated with radiation. Methods A retrospective study was done in 40 radiotherapy uncontrolled cases of squamous cell carcinoma of the cervix uteri and an equal number of cases, matched in age, clinical stage, tumor size and histologic grade, which had been cured by radiotherapy. Cell proliferation was judged by the proliferating cell nuclear antigen (PCNA) positively stained cell number × intensity of staining, and number of cells in mitosis per 10 high power microscopic fields (HPF). Apoptotic cells were identified by TUNEL method and the result was expressed as the number of cells undergone apoptosis per 10 HPF. Results The average level of PCNA expression was 5.38±3.21 in the cured patients and 7.50±3.16 in the radiotherapy uncontrolled patients ( P <0.01). The number of mitotic cells/10 HPF was 6.33±5.15 in the cured patients and 11.33±7.50 in the uncontrolled patients ( P <0.001). The number of apoptotic cells/10 HPF was 20.90±8.19 in the cured patients and 27.13± 17.28 in the uncontrolled patients ( P <0.05). The ratio of number of apoptotic cells to that of mitotic cells (A/M) was 4.97±3.31 in the cured and 3.36±2.83 in the uncontrolled ( P <0.05). Conclusion In cervical cancer, PCNA expression, the number of cells in mitosis and that of cells in apoptosis are significantly increased while the A/M ratio is significantly decreased in radiotherapy uncontrolled patients compared to those of patients cured by radiotherapy. These parameters may be of value to predict response to radiotherapy and prognosis of patients with cervical cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1999年第4期290-292,共3页
Chinese Journal of Oncology
关键词
宫颈肿瘤
放射疗法
细胞增殖
细胞凋亡
预后
Cervical neoplasms/radiotherapy Cell proliferation Apoptosis Prognosis Proliferating cell nuclear antigen
