摘要
目的 研究ＭＡＬ基因在人食管癌中的表达下调状况。方法 采用Ｎｏｒｔｈｅｒｎ 杂交和ＲＴＰＣＲ方法检测了ＭＡＬ基因在４１ 对配对的食管癌组织和相应癌旁食管粘膜中的表达；应用ＰＣＲ和ＲＴＰＣＲ方法分析了ＭＡＬ基因在３ 个食管癌细胞系ＥＣ１０９ 、ＥＣ８７１２ 和ＥＣ９７０６ 中的存在及其表达。结果ＭＡＬ基因的表达水平在９２７ ％ （３８／４１） 食管癌组织中明显低于同一患者的癌旁食管粘膜。同时，ＭＡＬ基因在３ 个食管癌细胞系中未见表达。ＰＣＲ分析显示，这３ 个食管癌细胞系中均存在被分析的ＭＡＬ基因片段。结论 ＭＡＬ基因表达下调是食管癌发生。
Objective To investigate the status of expression of MAL gene in human esophageal cancer. Methods Expression of MAL gene was analyzed by Northern blot and/or RT PCR in 41 pairs of human esophageal cancer tissues and matched adjacent normal mucosa, and three human esophageal cancer cell lines EC109, EC8712 and EC9706. MAL gene was analyzed by PCR in the three esophageal cancer cell lines.Results MAL gene expression was down regulated substantially or barely detectable in 93%(38/41) of human esophageal cancer tissues while expressed at high level in all matched adjacent normal esophageal mucosa. In all 3 human esophageal cancer cell lines, expression of MAL gene was not detectable. PCR analysis showed that the MAL gene fragment analyzed was intact in the three esophageal cancer cell lines.Conclusion Down regulation of MAL gene expression is of frequent occurrence in human esophageal cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1999年第4期250-252,共3页
Chinese Journal of Oncology
基金
863 重大项目基金
九五攻关项目基金
攀登项目资助
关键词
食管肿瘤
MAL基因
基因表达
聚合酶链反应
Esophageal neoplasms MAL gene Gene expression Polymerase chain reaction
