摘要
目的探讨小鼠腔前卵泡的最佳分离方法。方法取性未成熟小鼠的卵巢,按照不同分离方法,单纯机械法与酶解法分离腔前卵泡,并用基础培养基进行培养,比较不同分离方法获得的卵泡数量、形态,及对其早期生长发育的影响。结果2mg/mL的胶原酶可获得大量基底膜完整的腔前卵泡及裸卵(P<0.01);体外培养2d后,酶解组卵泡生长直径大(P<0.05)、GVBD率、放出第一极体率和7d生存率无明显差异(P>0.05)。结论胶原酶酶解法较单纯机械法更易获得大量高质量腔前卵泡,且对其早期发育无明显影响。
【Objective】To explore the optional isolation methods of preantral follicle.【Methods】The ovary was obtained from premature mouse and dissected according to different isolation methods, mechanical dissection or enzymatic digestion. Then they were cultured in the basal medium and the quantity, morphology and effects on early development were assessed. 【Results】2 mg/mL collagenase was the optional isolation condition to obtain large amount of preantral and denuded follicles （P 0.01）, and the follicles in enzymatic group grew faster than mechanical group （P 0.05）, but the GVBD rate, Polar body releasing rate and surviving rate have no obvious difference （P 0.05）. 【Conclusion】Collagenase isolation could obtain large amount of high quality preantral follicles than mechanical methods, which also had minimal effects on early development.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第5期695-698,共4页
China Journal of Modern Medicine
基金
卫生公益性行业科研专项(No:200802159)
高等学校博士点科研基金(No:200804870009)
国家自然科学基金面上项目(No:30973148)
关键词
小鼠
腔前卵泡
分离方法
体外培养
mouse
preantral follicle
isolation methods
in-vitro culture
