摘要
用174个SSR、41个EST、345个SNP标记对以镜鲤良种后代为祖父母本所培育的杂交F2群体的68个个体进行基因型检测,运用JoinMap4.0软件包构建遗传连锁图。利用MapQTL5.0区间作图法(interval mapping,IM)和多QTL区间定位法(MQM mapping,MQM)进行QTL检测,通过置换实验(1000次重复)确定连锁群显著性水平阈值。在对体高、头长、体厚的区间定位中,共检测到6个与体高性状相关的QTLs区间,分布在LG1(SNP1339-SNP1490)、LG10(HLJE469-SNP1491)、LG12(SNP0922-HLJ1316)、LG13(SNP0937-HLJ328)、LG25(SNP1041-HLJ594)、LG35(SNP1425-SNP0389)等6个连锁群上,解释表型变异范围为20.0%～43.3%。其中,SNP1339-SNP1490区间LOD值最大为3.64,解释表型变异35.4%。6个与头长相关的QTLs,分布在LG1(SNP1339-SNP1490)、LG12(HLJ071-HLJ336)、LG13(SNP0937-HLJ328)、LG24(SNP1359-SNP0586)、LG24(SNP1016-SNP0326)、LG25(HLJ382-HLJ348)等5个连锁群上,其中LG24(SNP1359-SNP0586)解释表型变异达到50.2%。检测到10个与体厚相关的QTLs,分布在LG1、LG8、LG9、LG10、LG12、LG34、LG35、LG38、LG41等9个连锁群上,解释表型变异范围是16.1%～68.8%,其中LG38(HLJ1331-HLJ487)和LG41(HLJ688-HLJE6)解释表型变异分别达到60.8%和68.8%,是体厚性状的主效QTLs区间。
A group of F2 hybrids common carp including 68 individuals was used to construct a linkage map using 560 markers（174 SSR markers,41EST-SSR markers and 345 SNP markers） .Quantitative traits loci （QTLs） associated with body hieght,head length,body thickness were identified by interval mapping and MQM Mapping of the software MapQTL5.0.A linkage group wide permutation test（1 000 replicates） determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group.Six QTLs were identified for body height on the linkage groups of LG1 （SNP1339-SNP1490 ） ,LG10 （HLJE469SNP1491 ） ,LG12 （SNP0922-HLJ1316 ） ,LG13 （SNP0937-HLJ328 ） ,LG25 （SNP1041-HLJ594 ） ,LG35 （SNP1425-SNP0389） ,which explained 20.0% to 43.3% of the total variation of the body height.The LOD value of SNP1339-SNP1490 is 3.64,which is the highest and explaining 35.4% of the total variation of the body height.Six QTLs were associated with on the linkage groups of LG1 （SNP1339-SNP1490 ） ,LG12 （HLJ071-HLJ336） ,LG13 （SNP0937-HLJ328） ,LG24 （SNP1359-SNP0586） ,LG24 （SNP1016-SNP0326） ,LG25（HLJ382-HLJ348） ,in which LG24（SNP1359-SNP0586） explained the total variation of the head lentgh reaching 50.2% .Ten QTLs were responsible for body thickness on nine linkage groups explaining 16.1% and 68.8% of the total variation of the body thickness respectively,in which LG38（HLJ1331-HLJ487） and LG41 （HLJ688-HLJE6） explaining 60.8% and 68.8% of the total variation of the body thickness respectively.They were major QTLs associated to the body thickness of common carp.
出处
《水产学报》
CAS
CSCD
北大核心
2010年第11期1645-1655,共11页
Journal of Fisheries of China
基金
水产分子育种共性技术的建立与应用(200903045)
关键词
鲤
头长
体高
体厚
数量性状定位
common carp （Cyprinus carpio）
head length
body height
body thickness
quantitative trait locus
