摘要
鲫(Carassius auratus)遗传多样性丰富,具多种不同倍性的亚种,是研究鱼类进化的独特材料。本研究利用79个SSR标记和22个EST-SSR标记检测了鲫自交F2代的181尾个体的基因型,对体重、体长、体高和体厚4个生长性状进行了单标记回归分析。Permutation检验(10000次)结果显示:有22个标记分别与体重、体长、体高和体厚具有显著相关性(P<0.05),其中,HLJYJ15和HLJYJ244与4个性状均呈极显著相关(P<0.01)。本研究筛选的101个微卫星标记丰富了鲫分子标记数量,可用于鲫连锁图谱的构建和QTL定位研究;获得了4个与鲫性状相关的标记及基因型,为鲫的分子标记辅助育种(MAS)和生长性状的遗传机制研究提供了工具。
Crucian carp Carassius auratus is rich in genetic diversity, and becomes a good candidate for research of molecular markers or genes associated with those growth traits. In the current study, 79 SSR and 22 EST-SSR markers were chosen to analysis the genom- ic of 181 progenies from a crucian carp F2 family. A GLM （General Linear Model） procedure was used to analyze the correlation be- tween markers and four growth traits including body weight, body length, body depth and body width. The significance testing by Per- mutation test（ 10 000 replicates）, 22 makers were shown to fit the correlation from four growth traits （P〈0.05）, very significant corre- lation （P〈0.01） for HLJYJ15 and HLYJ244. Markers and gene types related to the four growth traits were obtained from this research, which gives effective basis to the study of crucian carp marker-assisted breeding and genetic mechanism of growth traits.
出处
《水产学杂志》
CAS
2012年第2期1-6,共6页
Chinese Journal of Fisheries
基金
公益性行业(农业)科研专项(200903045)
国家973计划项目(2010CB126305)
农业部948计划项目(2011-G12)
关键词
鲫
SSR
EST-SSR
体重
体长
体高
体厚
Carassius auratus
SSR
EST-SSR
body weight
standard length
body depth
body width
