摘要
目的:探讨3-硝基丙酸(3-NPA)预处理对大鼠局灶性脑缺血半暗带区不同再灌注时点GLUT1和GLUT3mRNA及蛋白水平表达的影响。方法:雄性SD大鼠40只,随机分为假手术组(sham组,n=4)、预处理对照组(3-NPA组,n=4)、大脑中动脉缺血组(M组,n=16)、3-NPA预处理组(IPC组,n=16)。M组和IPC组按再灌注时间(4h、12h、24h及48h)不同又分为4个亚组,每组动物4只。将大鼠在相应时点断头取脑,取缺血侧(左侧)冠状面中间1/3皮质,分别采用RT-PCR和Western blotting方法检测GLUT1、GLUT3 mRNA和蛋白水平表达情况。结果:IPC组GLUT1 mRNA表达在缺血再灌注后4h开始升高,48h最大,显著高于sham组和M组相应时点。IPC组GLUT3 mRNA表达在24h增高,48h最高,与M组相应时点24h、48h及sham组比较显著增高。IPC组比M组的GLUT1蛋白、GLUT3蛋白表达增高,有显著差异(F=5.848,P<0.05;F=6.295,P<0.05),尤以缺血再灌注后48h两者差异最明显。结论:3-NPA预处理能诱导脑缺血耐受,其机制可能是上调GLUT1和GLUT3 mRNA及蛋白表达水平,维持脑组织的能量供给。
AIM： To explore the mechanism of 3-nitropropionic acid （3-NPA） preconditioning that induces cerebral ischemic tolerance in rats by affecting the expression of brain-type glucose transporters （GLUT1 and GLUT3） at mRNA and protein levels in cerebral tissues.METHODS： The male SD rats were used in the experiments and divided randomly into sham operation group （sham group, n=4）, control group of 3-NPA preconditioning （3-NPA group, n=4）, cerebral ischemia group （M group, n=16） and 3-NPA preconditioning group （IPC group, n=16）. M group and IPC group were further divided into 4 subgroups according to the different reperfusion time（4 h, 12 h, 24 h and 48 h）. All rats were killed at the corresponding time points. The cerebral tissues in the ischemic side （left） and coronal intermediate 1/3 of cortex were collected. The protein levels and mRNA expression of GLUT1 and GLUT3 were determined by Western blotting and RT-PCR. RESULTS： Compared with M group, the ischemic reperfusion and 3-NPA preconditioning induced the upregulation of GLUT1 and GLUT3 at protein levels with significant differences （F=5.848, P〈0.05 and F=6.295, P〈0.05, respectively）, especially after ischemia-reperfusion for 48 h. The mRNA expression of GLUT1 in IPC group began to increase at 4 h, peaked at 48 h after reperfusion, with significant difference as compared to M group at the corresponding reperfusion time points in each group or sham group. In contrast, the mRNA expression of GLUT3 in IPC group increased at 24 h, and was the highest at 48 h as compared to cerebral ischemia group at the corresponding reperfusion time points or sham group.CONCLUSION： 3-NPA preconditioning increases the expression of GLUT1 and GLUT3 at protein and mRNA levels to maintain the energy supply in brain tissues, indicating a cerebral protective mechanism.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第11期2212-2216,共5页
Chinese Journal of Pathophysiology
基金
温州市科技局资助项目(No.Y20070013)
关键词
脑缺血耐受
缺血预处理
3-硝基丙酸
葡萄糖转运蛋白质1
葡萄糖转运蛋白质3
Brain ischemia tolerance Ischemic preconditioning 3-Nitropropionic acid Glucose transporter protein 1 Glucose transporter protein 3
