摘要
目的 :了解大鼠脑缺血再灌注后通透性的变化及其与内皮细胞糖复合物的关系。方法 :用暂时性脑缺血的大鼠模型 ,以右旋糖酐分子为示踪剂对缺血再灌注后微血管通透性的变化进行了观察 ,同时用同一标本经K4M低温包埋 ,用阳离子胶体金包埋后标记的方法在超微结构显示缺血再灌注后内皮细胞表面糖复合物 (glycocalyx)的变化。结果 :电镜下见缺血 1h和再灌注后 1h、2h、3h、4h、6h、9h、12h各时点均可见右旋糖酐颗粒穿出血管外 ,胞浆内出现的大量吞饮小泡 ,细胞间连接的增宽和开放 ,内皮细胞胞膜表面的凹陷及多量微绒毛的出现是微血管通透性增高的结构基础 ,大鼠脑缺血后微血管通透性增高的同时阳离子胶体金分布减少。结论 :Glycocalyx对缺血/缺氧性损害极其敏感 。
AIM: To determine the role of the glycocalyx in microvascular permeability. METHODS: Dextran was intravenously injected and quatitatively examined in the rat transient cerebral ischemic model. At the same time, endothelial glycocalyx (anionic sites) was labelled with the probe cationic gold colloid (CGC) using post-embedding technique and examined with electron microscope. RESULTS: The labeling of CGC decreased significantly following ischemia, meanwhile, microvascular permeability to dextran increased. CONCLUSION:Endothelial glycocalyx is very sensitive to ischemia or anoxia. Its disruption may be the initiator of the dysfunction of endothelium and the determinant of increased permeability.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第8期718-721,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助课题 (No .39770 2 72 )
