摘要
目的:探讨黑色素瘤相关抗原H1(Mage-H1)在细胞增殖和分化调控中的作用。方法:神经生长因子(nerve growth factor,NGF)诱导PC12细胞分化后用相差显微镜对其形态学进行观察。通过RT-PCR、Western blotting检测分化前后Mage-H1表达变化情况。流式细胞技术分析PC12细胞瞬时表达Mage-H1对细胞周期的影响。结果:经NGF诱导8d后,92%以上PC12细胞属于已分化细胞。分化后的PC12细胞与对照组相比,Mage-H1表达上调。PC12细胞瞬时表达Mage-H1后被明显阻滞在G0-G1期。结论:Mage-H1可抑制PC12细胞增殖并促进细胞的分化。
AIM ： To explore the effects of melanoma - associated antigen HI （ Mage - HI ） on cell proliferation and differentiation. METHODS： A phase contrast microscope was used to observe the morphological changes of PCI2 cells treated with or without nerve growth factor （NGF）. The expression of Mage - H1 in pre - and post - differentiated PC12 cells was detected by RT- PCR and Western blotting, and its potential effects on the cell cycle were analyzed by flow cytometry. RESULTS： After induced by NGF for 8 days, over 92% of PCI2 cells were differentiated. The relative levels of Mage - H1 mRNA and protein in the differentiated PC12 cells were g. 6 times and 2. 6 times higher than those in control cells ,respectively. Moreover, the PC12 cells transiently expressed Mage- H1 were significantly arrested in G0 -G1 phase as compared to the cells transfected with an empty vector. CONCLUSION： Mage - HI inhibits the proliferation of PC12 cells and promotes the differentiation of the cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第11期2112-2117,共6页
Chinese Journal of Pathophysiology
