摘要
将鸡传染性支气管炎病毒肾型 Ｔ 株 Ｓ１ 基因ｃ Ｄ Ｎ Ａ 连接于ｐｃ Ｄ Ｎ Ａ３ 的 Ｈｉｎｄ Ｉ Ｉ Ｉ与 Ｂａ ｍ Ｈ Ｉ位点之间构建含有 Ｃ Ｍ Ｖ 启动子及 Ｂ Ｇ Ｈｐｏｌｙ Ａ 信号序列的鸡传染性支气管炎病毒 Ｓ１ 蛋白真核表达质粒。实验证明， Ｓ Ｐ Ｆ 鸡肌注免疫后血清 Ｉｇ Ｇ 抗体逐渐升高，至第３５ 日龄左右达到高峰，攻毒后血清 Ｉｇ Ｇ 抗体先下降而后升高，血清 Ｉｇ Ｇ 抗体升高幅度不及 Ｉ Ｂ 油苗免疫组。质粒 Ｄ Ｎ Ａ 免疫鸡攻毒后有４０ ％ 的鸡可耐过强毒的攻击，说明 Ｓ１ 基因在体内获得了表达并使鸡产生了一定的免疫力。
To construct the IBV S1 gene expression plasmid with CMV promoter and BGH polyA signal sequence,S1genl cDNA of Australia strain was inserted into BamHI/HindIII site of pcDNA3 plasmid.Results demonstrated that with the development of serum IgG,the peak of serum IgG could be observed 35 days post S1 DNA vaccination in SPF chickens.The changing of the IgG in DNA vaccinated chickens inoculated with IBV virulent strain rose first,then declined.The changing degree of DNA vaccination group was less than that of IBV vaccionation group.40% chickens immunized with S1 gene DNA vaccine can be protected aganst IBV infection.
出处
《中国预防兽医学报》
CAS
CSCD
1999年第4期250-253,共4页
Chinese Journal of Preventive Veterinary Medicine
