摘要
以吖啶为紫外检测试剂，研究了强极性弱紫外吸收磷霉素的间接光度色谱，探讨了检测试剂性质、浓度、pH值等因素对色谱保留和检测灵敏度的影响，提出一种测定磷霉素的新色谱方法。
Fosfomycin is a broad-spectrum antibacterial agent which has strong polarity and weak UV absorption. In this work, a method using standard ODS column and conventional UV detector set at 254 um to analyse fosfomycin was investigated. Firstly, several UV detection reagents were tested and acridine(A) was found to give the highest indirect detection response and proper modification on retention of fosfomycin. With acridine as the UV detection reagent, the mobile phase parameters which affect retention and detection response of solute are discussed, e. g. pH, concentration of organic solvent and concentration of acridine. The mechanism is interpreted for each case. After optimization, we adopted mobile phase as V(CH3OH): V(H2O) = 50: 50, pH 3. 18, [HAc] = 2 mmol/L, [A] =1 ×10-5 mol/L. A new chromatographic system monitored by indirect photometry for detection of fosfomycin was established. This method was used for quantitative analysis, the minimum detectable level was 10 μmol/L, the relative deviation was 4. 51 %.
出处
《色谱》
CAS
CSCD
北大核心
1999年第3期271-274,共4页
Chinese Journal of Chromatography
关键词
间接光度检测
HPLC
吖淀
磷霉素
测定
indirect photometric detection, high performance liquid chromatography,acridine, fosfomycin
