摘要
根据植物菌原体１６ＳｒＲＮＡ基因序列，合成一对寡核苷酸引物，用ＳＤＳＣＴＡＢ改进法直接提取田间发病的柿树带化病、刺槐丛枝病树和健康树皮层的ＤＮＡ，用ＰＣＲ方法成功地从病树ＤＮＡ模板中扩增出约１．１７ｋｂ的特异产物，经酶切进一步证实为植物菌原体１６ＳｒＤＮＡ．此项研究表明用特异合成的引物Ｆ１６／Ｒ１６，经ＰＣＲ扩增可以快速灵敏地检测植物菌原体．
A
pair of oligonucleotide primers was designed on the basis of Phytoplasmas 16SrRNA gene
sequences. Total DNA was extracted from persimmon fasciation, black locust witches' broom
and their healthy plant tissues collected from fields using amended SDSCTAB method. PCR
products at the length of 1.17kb amplified from phytoplasmainfected tissues DNA were
confirmed as Phytoplasmas 16SrDNA. This research indicated that Phytoplasmas could be
inspected rapidly and sensitively by Polymerase Chain Reaction (PCR) with a specific primer
pair F16/R16.\=
出处
《北京林业大学学报》
CAS
CSCD
北大核心
1999年第3期26-28,共3页
Journal of Beijing Forestry University
基金
国家自然科学基金
关键词
柿树带化
刺槐丛枝
PCR检测
persimmon fasciation, black locust witches'broom, PCR examination
