摘要
用不同的基本培养基、不同的植物生长调节剂及其配比、不同的原球茎切割方式系统地研究了黄花白芨（Ｂｌｅｔｉｌａｏｃｈｒａｃｅａ）组织培养技术。试验结果表明：１／２ＭＳ＋１０ｍｇ·Ｌ－１ＢＡ＋０１ｍｇ·Ｌ－１ＮＡＡ培养基有利于原球茎增殖，培养６０ｄ增殖到４２１倍；Ｋｙｏｔｏ＋１０ｍｇ·Ｌ－１ＢＡ＋０１ｍｇ·Ｌ－１ＮＡＡ的培养基有利于诱导原球茎增殖、分化及幼苗的生长，培养６０ｄ分化形成的芽数为接种原球茎数的４７９倍；Ｋｙｏｔｏ＋２０ｍｇ·Ｌ－１ＢＡ＋０１ｍｇ·Ｌ－１ＮＡＡ的培养基对球茎切块诱导芽的效果最好。在继代培养中，应采用纵切法切割球茎或用自然掰开法来分割原球茎。同时，对进一步的研究提出了建议。
A study of techniques for tissue cultures of Bletilla ochracea was made in
terms of basic culture media,hormones and their proportions and the cutting of original
bulbs.Its showed that the medium compose of 1/2 MS+10 mgL-1BA+01 mgL-1NAA was
favorable to the proliferation of the original bulb,with 421 times asmany as thd original
proliferated in 60 days;the medium consisting of Kyoto+10 mgL-1BA+01 mgL-1NAA was
favorable to the induction of the original bulb to proliferate and differentiate and the
growth of seedlings,with 479 times as many as the number of buds of the original bulb
differentiated and formed within 60 days;and the medium with Kyoto+20 mgL-1BA+01
mgL-1NAA involved had a good effect on the bud induction of pieces cut from the
original bulb.In the culture of subsequent generations,vertical cutting of the bulb or
natural separation of the original bulb should be used.Some suggestions for further
studies are put forward meanwhile.
出处
《浙江林学院学报》
CSCD
1999年第2期164-169,共6页
Journal of Zhejiang Forestry College
关键词
黄花白芨
组织培养
原球茎
繁殖
分化
Beltilla ochracea
tissue
cultures
protocorm propagation
differentiation(biology)
