摘要
目的:为适时开展白及种子成苗技术提供技术支撑。方法:采用沙藏、自然贮藏、低温干藏方法分别贮藏白及种子120、150、180 d常规种子皿内发芽法测定萌发率,SSR法比较差异性。结果:白及种子有胚率(84.9±4.7)%、新鲜种子萌发率(92.1±2.1)%;沙藏、自然贮藏、低温干藏种子120、150、180 d的平均萌发率分别为(89.3±2.7)%、(77.4±3.3)%、(68.9±2.7)%,相互间呈显著或极显著差异;种子贮藏120、150、180 d时,三种方法的平均萌发率分别为(81.7±8.0)%、(78.5±7.9)%、(75.7±8.5)%,其中贮藏120 d与180 d的萌发率呈显著差异;新鲜与沙藏120 d白及种子的萌发率无显著差异。结论:沙藏白及种子的萌发效果好,贮藏180 d仍有较高萌发率。
Objective: Exploring seed storage method to offer a technological support for the seedling development of Bletilla striata.Methods: Bletilla striata seeds were stored by means of sand storage,nature storage and low temperature storage for 120,150 and 180 d respectively.The germination rates were measured with methods of seed germination on the Petri dish and difference of rates was compared by means of the SSR method.Results:The embryo rate of Bletilla striata seeds was(84.9±4.7)%;the germination rate of fresh seeds was(92.1±2.1)%;the germination rates of seeds treated by sand storage,nature storage and low temperature storage for 120,150 and 180 d were(89.3±2.7)%,(77.4±3.3)%,(68.9±2.7)% respectively and they showed significant difference or extreme significant difference one another.When seeds stored 120,150 and 180 d,germination rates of seeds treated with three methods were(81.7±8.0)%,(78.5±7.9)%,(75.7±8.5)% respectively,and in these three storage times,germination rates of seeds stored 120 d and 180 d showed significant difference.In addition,there was no significant difference between germination rates of fresh seeds and sand storage seeds stored 120 d.Conclusion:The effect of germination of seeds stored with sand was good,and germination rate was high steal after 180 d.
出处
《中药材》
CAS
CSCD
北大核心
2010年第1期7-10,共4页
Journal of Chinese Medicinal Materials
基金
重庆市医学科学研究项目(20091053
2009-B-47)
重庆市自然科学基金项目(2009B5252)
关键词
白及
贮藏
种子
萌发
Bletilla striata(Thunb.) Reichb.f.
Storage
Seed
Germination