摘要
目的 观察色素上皮衍生因子（PEDF）在前列腺癌发生发展中对前列腺癌抑制的作用.方法 通过免疫组化S-P法及Western印迹检测正常前列腺组织、良性前列腺增生组织、前列腺癌组织以及前列腺癌细胞系PC-3、Lncap中PEDF的表达情况,结合临床资料进行统计分析,评价PEDF表达情况与前列腺组织癌变之间的关系 同时联系前列腺癌不同病理分级,并研究其与PEDF表达之间的关系.结果 在正常前列腺组织及良性前列腺组织中PEDF均处于高表达状态,远远高于前列腺癌组织及前列腺癌细胞系中PEDF的表达[7例正常前列腺组织中7例PEDF阳性表达 20例良性前列腺增生组织19例PEDF阳性表达 83例前列腺癌组织中24例（28.9%）PEDF阳性表达].PEDF的表达情况与前列腺癌病理分级相关,低分化的前列腺癌组织中PEDF的表达量低于高分化的前列腺癌组织.在转移的前列腺癌组织中PEDF的表达率为12%,低于未转移的前列腺癌组织中的43.1%.结论 PEDF与前列腺癌的发生呈负相关,PEDF表达量越低前列腺癌细胞的恶性度越高,越具有转移侵袭的能力.
Objective To study the inhibition and significance of pigment epithelium-derived factor （PEDF） in the development and metastasis of prostate cancer. Methods The expression of PEDF was examined in the normal prostate tissue, benign prostatic hyperplasia, prostate cancer tissue and prostate cancer cell lines, PC-3 and Lncap by immunohistochemical SP method and Western blot In combination with clinical data, statistical analysis was performed to evaluate the relation of the expression level of PEDF in prostate cancer and the relationship between different histological grades of prostate cancer. Results In normal prostate tissue and benign prostate tissue, the expression of PEDF were elevated and it was far higher than the prostate cancer and prostate cancer cell line. The expression of PEDF and the pathological grade of prostate cancer were related to the differentiated carcinoma of prostate tissue, and the expression level of PEDF in poorly differentiated carcinoma below the highly differentiated carcinoma of prostate cancer. In metastatic prostate cancer, the expression of PEDF was lower than that of prostate cancer without metastasis （12% vs 43. 1%）. Conclusion The expression of PEDF and the incidence of prostate cancer have a negative correlation. The lower grade of prostate cancer cells, the less the tissue expression of PEDF and the higher tendency of invasion and metastasis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第42期2980-2983,共4页
National Medical Journal of China
基金
基金项目：广东省医学科学基金（A2008178）
关键词
前列腺肿瘤
色素上皮衍生因子
转移
免疫组化
Prostate neoplasms Pigment epithelium-derived factor Metastasis Immunohistochemistry
