摘要
目的:探讨体外培养的股中间肌卫星细胞对大鼠骨骼肌钝挫伤的修复作用,为提高对肌肉损伤的治疗提供参考依据。方法:分别取体外培养SD大鼠股中间肌卫星细胞悬液(实验组)与生理盐水(对照组),注入大鼠骨骼肌钝挫伤模型损伤部位,观察比较两组动物损伤修复进程及差异。结果:移植后实验组比对照组恢复效果好。(1)卫星细胞移植后5、10、15天,实验组肌肉组织内荧光标记的肌卫星细胞逐渐减少,形成肌管并最终融合成肌纤维参与损伤修复;(2)移植后5、10、15天大鼠肌电图的纤颤电位和正尖波均逐渐减少,而实验组大鼠异常自发活动的消失较对照组早,且损伤侧肌肉肌电强度振幅和波宽依次增大,逐渐接近正常侧,而对照组肌电强度振幅与波宽则增加缓慢;(3)随着损伤修复的进程,实验组肌肉湿重恢复率逐渐降低并接近1;(4)随着修复的进程,HE染色灰度值逐渐增加,且实验组灰度值大于对照组。结论:股中间肌卫星细胞移植对骨骼肌钝挫伤有明显修复作用。
Objective To investigate the effect of transplantation of the femoral muscle satellite cells on the muscle contusion.Methods Muscle contusion model was developed in 36 SD rats,and the rats were divided into experimental group and control group.Femoral muscle satellite cells and physiological saline were implanted into contused position of experimental group and control group,respectively.The reparing process of skeletal muscle between the two groups was observed.Results（1）5,10,and 15 days after the transplantation of satellite cells：the number of fluorescence labeled satellite cells in muscle tissue reduced gradually,and myotubes were formed and eventually fused into muscle fibers in the experimental group;the fibrillation potentials and positive sharp waves in EMG were progressively reduced,while the abnormal spontaneous activity in the experimental group disappeared earlier than in the control group,and the EMG amplitude and pulse width increased in turn and gradually got close to the uninjured side,while the EMG amplitude and pulse width in control group increased slowly.（2）With the progress of the muscle repair,the recovery rates of muscle’s wet weight were progressively reduced,and the gray values of HE staining were gradually increased in experimental group as compared with the control group.Conclusion The transplantion of femoral muscle satellite cells into the contused muscles can significantly accelerate the repair process.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2010年第6期697-701,共5页
Chinese Journal of Sports Medicine
基金
南通市科技局社会发展科技计划资助项目(S2008022)
关键词
股中间肌卫星细胞
骨骼肌
钝挫伤模型
移植
修复
femoral muscle satellite cell
skeletal muscle
contusion mode
transplantation
repair
