摘要
以甜瓜抗白粉病品种‘银帝’和感白粉病品种‘卡拉克赛’为材料,研究诱导抗性处理对其叶片酚类物质代谢的影响及其与抗病性的关系。结果表明:苯丙噻重氮(BTH)和水杨酸(SA)处理以及接种白粉菌均可显著诱导甜瓜叶片苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)活性和绿原酸、总酚、类黄酮含量升高,但纳米硅(SiO2)诱导无效。经诱导和接菌后,甜瓜抗病品种叶片PAL、POD、PPO活性和总酚、绿原酸、类黄酮含量高于感病品种。BTH和SA诱导甜瓜抗病性增强的原因之一是提高了叶片次生代谢相关酶的活性,增加了叶组织次生代谢物质的含量。
To investigate the effects of disease inducing treatment on phenolic metabolism of melon leaves and the relationship to leaf disease resistance,two melon cultivars（＇Yindi＇and＇Kalakesai＇） of different disease resistance were employed.Benzo-1,2,3-thiadiazole-7-carbothioic acid S-methyl ester（BTH）,salicylic acid（SA） and Nano silicon（SiO2）were sprayed five days before inoculation with spore suspension of Sphaerotheca fuliginea,phenolic compound contents and relative enzyme activities were determined as the indicators.The activities of phenylalanine ammonia lyase（PAL）,peroxydase（POD）,polyphenol oxidase（PPO）,and the contents of total phenols,chlorogenic acid,flavonoids in melon leaves increased significantly after spraying with BTH,SA and/or inoculation of Sphaerotheca fuliginea spore suspension.SiO2 spraying did not show such inducing effect.The contents of phenolic compounds and the activities of relative enzymes in the disease resistant cultivar were higher than those in the susceptible cultivar after inducing treatment.The results suggest that one of the possible mechanisms of BTH and SA spraying on induced resistance of melon leaves is via increased contents of phenolic compounds and enhanced activities of phenol-related enzymes.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第11期1759-1766,共8页
Acta Horticulturae Sinica
基金
国家科技支撑计划项目(2007BAD88B07)
甘肃省农业生物技术研究与应用开发项目(GNSW-2006-06)
关键词
甜瓜
白粉病
BTH
SA
SIO2
酚类代谢
Cucumis melo
powder meldew
BTH
SA
nano silicon
phenol metabolism
