摘要
目的:探讨猪小肠黏膜下层(SIS)作为组织工程肌腱支架的可行性。方法:将培养的肌腱细胞以5×106/mL的密度接种到经过处理的SIS卷轴上,形成细胞-支架复合物。24只罗曼鸡随机均分为实验组和对照组。2组动物均在左腿中趾切断趾深屈肌腱造成2cm的缺损,实验组植入细胞-支架复合物,对照组中将切断的自体肌腱原位缝合。于术后第3、6、9周分别从实验组和对照组随机取出4只动物,并进行大体形态、组织形态学、体式显微镜观察和生物力学测定。结果:2组所有鸡趾伤口术后2周均愈合。组织学检查显示,实验组中3周时胶原纤维数量相对少,排列紊乱;6周时细胞数量增多,形态也略显规则;9周时纤维数量多且排列规则。对照组为正常的肌腱纤维组织。生物力学测定证实9周后2组植入物抗拉力强度,差异无统计学意义(P>0.05)。结论:以SIS与肌腱细胞构建的肌腱组织能够达到替代肌腱缺损的要求,SIS可作为肌腱组织工程的一种理想的支架材料。
Objective： To investigate the feasibility of the small intestinal submucosa（SIS） as a scaffold for tissue engineering tendon. Methods： The cells were seeded in the processed SIS for 5×106/mL, forming the cell-seeded SIS. Twenty-four Roman chickens （12 weeks old） were randomly divided into experimental group and control group. The profound flexor tendons of the left legs were dissected for 2 cm in chickens of two groups. The defects were repaired by the cell-seeded SIS in chickens of experimental group. The autotendons were sutured to the defect in chickens of control group. The chickens were sacrificed at 3, 6, or 9 weeks after surgery. The specimens were detected including general appearance somato-typemicroscope, histological pattern and mechanical test. Results： The wounds were healed in all chickens of 2 groups. The results of histological examination showed that few fibers and the confused alinement of fibers at the 3rd week in chickens of experimental group. The alinement of fibers became a certain sequence at the 6th week. The increasing amount of fibers was seen and spread regularly at the 9th week. Normal tendon fibres were seen in the control group. There was no significant difference in mechanical strength at the 9th week in the two groups （P 0.05）. Conclusion： Cell-seeded SIS scaffold may be the suitable substitution for the defected tendons. SIS can be used as an ideal scaffold in the tissue engineering tendon.
出处
《天津医药》
CAS
北大核心
2010年第11期993-995,1027,共4页
Tianjin Medical Journal
关键词
肠黏膜
肌腱病
组织工程
组织支架
猪
intestinal mucosa tendinopathy tissue engineering tissue scaffolds swine
