摘要
在表型性状数值分析和ＡＦＬＰ指纹图谱分析的基础上，选取５４株斜茎黄芪根瘤菌的代表菌株及已知根瘤菌参比菌株，进行１６ＳｒＤＮＡ和２３ＳｒＤＮＡ的ＰＣＲ－ＲＦＬＰ比较分析。结果表明斜茎黄芪根瘤菌具有极大的系统发育多样性，分别具有２４个１６ＳｒＤＮＡ遗传图谱类型和２２个２３ＳｒＤＮＡ遗传图谱类型，１６ＳｒＤＮＡ与２３ＳｒＤＮＡＰＣＲ－ＲＦＬＰ聚类分析树状图谱有较好的一致性，但也存在一些差异。在对较大类群的划分上，它们的结果与表型性状数值分析结果有较好的一致性。将１６ＳｒＤＮＡ和２３ＳｒＤＮＡＰＣＲ－ＲＦＬＰ分析数据合并在一起进行分析时，得出２６个综合遗传图谱类型和１个综合聚类分析树状图谱。很明显，１６ＳｒＤＮＡ与２３ＳｒＤＮＡ的合并，能够得出更可靠的系统发育结论。
16S rDNA and 23S rDNA PCR-RFLP were applied to 54 rhizobial stiains isolated from Astragalusadeurgens and reference strains of some recognized rhizobial species based on the results of numerical analysis ofphenotypic characteristics and AFLP fingerpiniting.The results showed there was a great phylogenetic diversityamong the symbionts of Astragalus adsurgens. The RFLP analysis resulted in 24 and 22 different genotypes for16S rDNA and 235 rDNA respectively. In general, dendrograms derived from 16S rDNA and 23S rDNA PCR-RFLP correlated well, with only a little difference. For some large groups,the results of 16S rDNA and 23SrDNA PCR-RFLP were in good agreement with that of numerical analysis of phenotypic characteristics. Thecombined data from 16S rDNA and 23S rDNA PCR-RFLP analysis described 26 genotypes and one dendrogram.Obviously , the combination of 16S rDNA and 23S rDNA can get more reliable phylogenetic conclusion .
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第2期120-125,共6页
Microbiology China
基金
国家自然科学基金委与欧共体资助
关键词
16SrDNA
23SrDNA
PCR
斜茎黄芪
根瘤菌
16S rDNA, 23S rDNA,PCR-RFLP, Astragalus adsurgens, Rhizobia.This work were funded by European Union and the National Natural ScienceFoundation of China. (No .3967001 7), (No. IC18-CT96-0103)
