摘要
普通结瘤基因（ｎｏｄＡＢＣ）是所有根瘤菌所特有的、最为保守的基因，用苜蓿根瘤菌结瘤基因（ｎｏｄＡＢＣ）和豌豆根瘤菌的（ｎｏｄＣ）基因片段为探钉，与５２株包括常见土壤细菌、已知根瘤菌、根瘤未知分离物的总ＤＮＡ进行斑点杂交，探索用普通结瘤基因（ｎｏｄＡＢＣ）或（ｎｏｄＣ）探针鉴定根瘤菌的可能性。结果，未找到合适实验条件，使来自这两个种的结瘤基因只能与根瘤菌菌株杂交，而不与土壤细菌的菌株杂交。但在高温条件下，两种探针都专一性的和种内菌株杂交。此结果表明：在一定的实验条件下，普通结瘤基因探针用做根瘤菌的鉴定，只能在种的水平上，而不能在种以上的水平进行。
Fragments of nodABC genes from R.mcliloti and fragments of nodC genefrom R.leguminosarum were used as probes to study the possibility of identifying rhizobialstrains. Fifty-two strains were tested. They were root-and stem-nodulating bacteria and soilG￣-bacteria.The conditions which both of the probes could hybridize with all the rhizobialstrains without false reaction were not found. The gene probes just hybridized with thestrains from the same species acuratly under the limitted condition.It suggested that probesof common nodulation genes just could identify rhizobia in species level.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第6期323-327,共5页
Microbiology China
基金
国家自然科学基金
关键词
根瘤菌
结瘤基因探针
鉴定
Probe, Common nodulation genes,Identification, Rhizobium
