摘要
我们利用水稻苯丙氨酸解氨酶启动子(AQ630)和吲哚乙酰胺赖氨酸酯合成酶基因(iaaL)构建了维管束特异表达的植物表达载体pBAL1并导入烟草基因组中。比较了对照植株、转基因植株的幼茎和根外植体在组织培养中分化能力的变化,结果表明转基因植株茎外植体的不定芽形成明显受到促进,而转化植株根外植体在不定根发生方面对外源IAA的敏感性下降。
A vascular bundles specific expressing vector pBAL1 with a promoter AQ630 from rice phenylalanine ammonialyase gene and a gene encoding indoleacetic-lysine synthytase from Pseu-domonas syringae subsp. savastanoi was constructed. Affirmed by Southern blotting and RT-PCR analysis, the AQ630-iaaL transgenic plants
show increasing shoots-regeneration frequency of young stem explants on hormone-free 1/2 MS medium and lower sensibility to IAA when roots were induced from the root explants on the media containing different concentrations of IAA compared to untransformed plants.
出处
《实验生物学报》
CSCD
1999年第1期62-68,共7页
Acta Biologiae Experimentalis Sinica
关键词
LaaL基因
维管束
烟草
茎
根
分化
生长素
Nicotiana tabacum. Vascular bundles specific expression. IaaL gene. Transgenic plants. Tissue culture
