摘要
目的：在酶标板上进行ＤＮＡ探针杂交，评价其在检测临床标本中结核杆菌的应用价值。方法：收集７１份结核性标本和３２份非结核性标本，进行ＤＮＡ扩增，把扩增产物分别进行电泳和ＤＮＡ探针杂交，比较其结果。结果：７１份结核性标本电泳法阳性率为４５１％，ＤＮＡ探针法阳性率为６９０％；３２份非结核性标本二者均为阴性。结论：ＤＮＡ探针杂交可提高临床标本中结核杆菌的阳性检出率，是结核病早期诊断的一种新方法。
Objective: To assess the value of PCRDNA probe in detecting mycobacterium tuberculosis in clinical samples. Methods: To select 71 samples with tuberculosis and 32 samples with notuberculosis,they were detected by PCRelectrophoresis and PCRDNA probe.The results were compared. Results:The positive rates of 71 tuberculosis samples were 45.1% and 69.0%,respectively.32 notuberculosis samples were all negative. Conclusion: DNA probe could increase positive rate of detecting mycobacterium tuberculosis in clinical samples.It is a new method of diagnosing Tuberculosis.
出处
《河南医学研究》
CAS
1999年第2期174-175,共2页
Henan Medical Research
关键词
DNA探针
结核杆菌
PCR
结核病
诊断
DNA probe
mycobacterium tuberculosis
polymerase chain reaction
