摘要
目的通过胆道口括约肌（ｓｐｈｉｎｃｔｅｒｏｆＯｄｄｉ，ＳＯ）细胞培养及形态学观察，为胆道口括约肌的功能结构研究提供新的途径．方法通过对纯种兔ＳＯ的解剖基础研究，采用原代细胞培养技术（复合酶消化法和组织块培养法）进行体外ＳＯ细胞培养，免疫细胞化学和电镜鉴定，并重点对细胞骨架进行了观察．结果利用兔ＳＯ“壁外型”的特点，成功进行了ＳＯ平滑肌细胞的培养，其纯度＞９０％，细胞骨架具有平滑肌细胞的典型特征．结论兔胆道口括约肌可以进行纯度较高的培养，从而为进一步的ＳＯ相关研究提供了新方法．
AIM To study the
dyskinesia of Oddi’s sphincter in rabbits. METHODS Through topographic study of pure bred
rabbit sphincter of Oddi (SO), and primary culture methods including tissue block and combined
enzyme digest method, SO cells were cultured, and identified by immunocytochemistry and
electron micrography. RESULTS SO cells were successfully cultured on the basis of SO
“extra duodenal” characteristics, with a purity of more than 90%. Cellular skeleton had the
characteristics of smooth muscle cells. CONCLUSION Culture of SO cells may be a new way to
study the structure and function of SO.
出处
《世界华人消化杂志》
CAS
1999年第4期316-319,共4页
World Chinese Journal of Digestology
关键词
胆道括约肌
细胞培养
细胞骨架
sphincter of Oddi
cell, cultured
cytoskeleton
