摘要
目的设计可位点特异切割丙型肝炎病毒（ＨＣＶ）５’非编码区（５’ＮＣＲ）和Ｃ区的多位点核酶（ＲＺ）．方法根据“锤头结构”ＲＺ的设计原理，以ＨＣＶＨ（１ａ）株５’ＮＣＲ和Ｃ区ＲＮＡ为靶序列，计算机预测其二级结构，运用能量最低化程序，选择理论上理想的ＲＺ切割位点，并比较５株ＨＣＶ序列ＲＺ切点两翼的同性．结果在ＨＣＶ５’ＮＣＲ和Ｃ区１２４个自然切割位点（ＣＵＸ和ＧＵＸ）中选出２１３（ＣＵＣ），２６０（ＧＵＡ），４０７（ＧＵＣ）和４９８（ＣＵＵ）４个切割位点，不同株之间切点两翼ＲＺ结合序列同源性为１００％．结论计算机可作为抗病毒ＲＺ设计的辅助工具；ＨＣＶ上述４个位点可能是最理想的切割位点．
AIM To design the hammerhead multi unit ribozymes
(RZ) which may specifically cleave 5'noncoding region (5'NCR) and core region of hepatitis C
virus (HCV) RNA. METHODS The possible secondary structures of RNA sequence of HCV H
(1a) strain which was taken as the targeting RNA was predicted, the ideal cleavage sites were
selected, and their ribozymes were designed with computer according to the principle of the
“hammerhead structure” RZ and the lowest energy. And then the homogenecity of the
sequence (7-8nt) around the cleavage sites was compared with other 4 HCV strains. RESULTS
The four sites 213 (CUC), 260 (GUA), 407(GUC) and 498 (CUU) were selected from the 124
natural sites (CUX and GUX) in 5'NCR and C region of HCV H RNA. The sequences (7-8nt)
around the four sites which could be bound by their ribozymes were homogenous among 5 HCV
strains. CONCLUSION Computer may be used as a helpful aid for designing the ribozymes
against various virus RNA or mRNA. The 213, 260, 407 and 498 in 5'NCR and C region of HCV
RNA may be the most ideal cleavage sites.
出处
《世界华人消化杂志》
CAS
1999年第4期300-302,共3页
World Chinese Journal of Digestology
基金
全军医药卫生科研基金
