摘要
目的研究ｍｄｍ２基因在原发性肝细胞癌（ＨＣＣ）中的表达并探讨其与ｐ５３基因突变的关系．方法用银染ＰＣＲＳＳＣＰ法检测ｐ５３基因第５～８外显子的突变，原位杂交检测ｍｄｍ２基因ｍＲＮＡ的表达，ＳＡＢＣ法检测ｍｄｍ２蛋白的表达．结果３９３％（１１／２８）的病例有异常的电泳迁移率．ｐ５３基因突变与肿瘤的大小、分化及转移无关．原位杂交显示９例ＨＣＣ出现ｍｄｍ２基因ｍＲＮＡ增加，７例ＨＣＣ可检测到ｍｄｍ２蛋白表达，ｍｄｍ２基因表达与ＨＣＣ的大小、分化及是否转移无关．Ⅰ～Ⅱ级ＨＣＣ中ｍｄｍ２阳性表达率（１３３％）明显低于Ⅲ～Ⅳ级ＨＣＣ中的阳性表达率（５３８％）．１１例有ｐ５３基因突变的ＨＣＣ中，只有３例出现ｍｄｍ２基因表达，另外６例有ｍｄｍ２过表达的ＨＣＣ未见ｐ５３基因突变．ｐ５３基因突变的ＨＣＣ与ｐ５３基因无突变的ＨＣＣ相比，ｍｄｍ２基因表达阳性率无显著差别．结论ｐ５３基因突变和ｍｄｍ２基因表达在原发性ＨＣＣ的发病中起重要作用．ｍｄｍ２基因表达与ＨＣＣ的恶性程度相关．ｍｄｍ２基因表达与ｐ５３基因是否突变无关．
AIM To estimate the role of
mdm2 gene overexpression in primary hepatocellular carcinoma (HCC) and its relationship to
p53 gene mutation. METHODS Silver staining PCR SSCP was used to determine exon 5-8
mutation of p53 gene. In situ hybridization (ISH) was employed to detect the mdm2 gene
overexpression at mRNA level. Strept avidin biotin enzyme complex method was used for
immunohistochemical detection. RESULTS Of the 28 HCC cases examined, 11 (39 3%)
showed abnormal band mobility shifts, suggesting the presence of mutations in these exons.
No abnormal band shift was found in the noncancerous adjacent liver tissues. p53 gene
mutation had no significant correlation to the size, differentiation or metastasis of HCC. Nine out
of 28 (32 1%) cases were positive for mdm2 gene transcripts by ISH. About 7 of 28 (25%)
cases showed visibly detectable signal for mdm2 protein. Two cases with ISH positive failed
to show mdm2 immunoreactivities. The overexpression of mdm2 gene had no correlation
with the size, differentiation or metastasis of HCC, but in 15 grade Ⅰ-Ⅱ HCC, 2 (13 3%)
harbored mdm2 gene overexpression. It is much lower than that in grade Ⅲ-Ⅳ ( P <0 05),
in which 7/ 13 (53 8%) cases possessed mdm2 gene overexpression. Three out of 11
cases with p53 gene mutation demonstrated overexpression of mdm2 gene whereas the
remaining 6 cases which showed mdm2 gene overexpression had no p53 gene mutation.
There was no correlation between mdm2 gene overexpression and p53 gene mutation.
CONCLUSION p53 gene mutation and mdm2 gene overexpression are involved in
pathogenesis of HCC. mdm2 expression is associated with the severity of tumors.
Overexpression of mdm2 gene is not correlated with mutation of the p53 gene.
出处
《世界华人消化杂志》
CAS
1999年第4期291-292,共2页
World Chinese Journal of Digestology
关键词
肝肿瘤
MDM2基因
P53基因
基因突变
liver neoplasms
carcinoma, hepatocellular
mdm2 gene
p 53 gene
gene
expression
