摘要
建立了多壁碳纳米管糊电极测定盐酸甲氧氯普胺(MCPM)的电化学分析方法,研究了MCPM与牛血清白蛋白(BSA)相互作用的电化学行为。在pH2.2的Britton-Robinson(B-R)缓冲溶液中,MCPM在1.084V处出现一灵敏的氧化峰。电化学测定MCPM的线性范围为0.17～10.3mg/L,检出限为0.03 mg/L。对5.18mg/L的MCPM进行11次平行测定,相对标准偏差为2.4%。当BSA加入到MCPM溶液中,MCPM的氧化峰电流明显降低。其降低值(ΔIp)与BSA的浓度在5.00×10-7～1.67×10-5mol/L范围内呈良好线性关系,检出限为2.0×10-7mol/L。MCPM与BSA相互作用的结合常数为1.05×105L/mol。
A novel electrochemical method was developed for the determination of metoclopramide hydrocbloride on multi-walled carbon nanotubes paste electrode （MWNT -PE）. The interaction be- tween metoclopramide hydrochloride and bovine serum albumin （BSA） was investigated by cyclic voltammetry, differential pulse voltammetry and spectroscopic methods. In pH 2.2 Britton - Robinson （B -R） buffer solution, an irreversible oxidation peak of metoclopramide hydrochloride was observed on the MWNT - PE at 1. 084 V and its electrochemical response was greatly enhanced compared with that of on the carbon paste electrode. The anodic peak current was linear with the concentration of metoclopramide hydrochloride in the range of 0. 17 - 10. 3 mg/L with a detection limit of 0.03 mg/L. The relative standard deviation（ n = 11 ） for 5.18 mg/L metoclopramide hydrochloride was 2. 4%. In the presence of BSA, the redox peak current of metoclopramide hydrochloride was decreased and the decreasing value was linear with the BSA concentrations in the range of 5.00 × 10^-7 -1. 67× 10^-5 mol/L, and the detection limit was 2. 0× 10^-7 mol/L. The binding constant of interaction between metoclopramide hydrochloride and BSA was 1.05 × 10^5 L/mol.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第11期1126-1131,共6页
Journal of Instrumental Analysis
基金
陕西省自然科学基础研究计划资助项目(2010JM2020)
延安大学科研资助项目(YD2008-37)
关键词
电化学
多壁碳纳米管糊电极
盐酸甲氧氯普胺
相互作用
electrochemistry
multi-walled carbon nanotube paste electrode
metoclopramide hydro- chloride
interaction
