摘要
目的：探讨紫杉醇增敏放射对胶质母细胞瘤细胞凋亡和增殖活性的影响。方法：采用人胶质母细胞瘤细胞系ＢＴ３２５。①细胞经紫杉醇和（或）照射处理后，每６ｈ收集一批细胞，至４８ｈ结束。行流式细胞术细胞凋亡分析。②细胞经紫杉醇和（或）照射处理后，培养４ｄ，作嗜银染色、ＡｇＮＯＲ计数。结果：照射后２４ｈ出现凋亡率高峰；紫杉醇加照射组的凋亡率高于单纯照射组，尤其是较低剂量照射（２和４Ｇｙ）时，相差更为显著。对照组、紫杉醇组、照射组和紫杉醇加照射组平均每个细胞的ＡｇＮＯＲ数分别为２２．８、１６．２、１５．５和６．４。结论：紫杉醇能促进放射诱导的胶质母细胞瘤细胞凋亡。紫杉醇增敏放射后残存肿瘤细胞的增殖活性显著下降。
Objective:To determine the influnce of taxol radiosensitization on apoptosis and proliferation of glioblastoma.Methods: 1. The cells treated with taxol or / and radiation (RT) were harvested at 6 h intervals within 48 hours. Flow cytometry was performed to estimate the percentage of apoptosis. 2. The cells treated with taxol or / and RT were incubated for 4 d, and then silver staining and AgNORs count were performed.Results: The peak incidence of apoptosis occurred at 24 h after RT. The percentage of apoptosis in taxol plus RT group was higher than that in only RT group, especially when cells were irradiated with 2 to 4 Gy. The numbers of AgNORs / cell were 22.8 in control, 16.2 in taxol, 15.5 in RT and 6.4 in taxol plus RT group respectively. Conclusion: Taxol may enhance radiation induced apoptosis. Proliferation of glioblastoma cells can be inhibited significantly by RT with taxol.
出处
《军医进修学院学报》
CAS
1999年第2期95-97,共3页
Academic Journal of Pla Postgraduate Medical School
