摘要
目的研究不同年生人参、西洋参栽培土壤中微生物种群结构差异。方法采用RAPD技术对采集自吉林省抚松县的18份人参、西洋参根际土和根围土中的微生物种群结构进行遗传多样性分析。结果 栽培年限对根际土及根围土中的微生物种群结构有显著影响;人参和西洋参栽培土壤中的微生物种群结构也存在差异;寄主植物对土壤微生物种群结构的影响表现有根际效应。结论人参或西洋参根系分泌物对土壤微生物的定向选择压力可能是造成土壤微生物种群遗传多样性变化的主要原因之一,人参或西洋参栽培土壤中微生物种群结构变化是导致土壤生态功能紊乱以及连作障碍形成的关键因子。
Objective To study rhizosphere and non-rhizosphere soil microbial population genetic diversity of Panax ginseng and P. quinquefolium with different growing years. Methods Eighteen P. ginseng and P. quinquefoliurn rhizosphere and non-rhizosphere soil samples were collected from Fusong county of Jilin Province, and genetic diversity of microbial population construction were analyzed by RAPD. Results Cultivation times have great influence on microbial population construction of rhizosphere and non-rhizosphere soil. Also we found that there were differences between soil microbial population construction of P. ginseng and P. quinquefoliurn. Furthermore, rhizosphere effect of plants on soil microbial population construction was also found. Conclusion Directional selection pressure that root secretions of P. ginseng and P. quinquefolium to soil microorganisms is one of the main drives that resulted in the change of microbial population genetic diversity, and microbial population construction change in P. ginseng and P. quinquefolium cultivated soil is the key factor for soil ecological function turbulence and the development of continuously cropping obstacle.
出处
《中草药》
CAS
CSCD
北大核心
2010年第11期1871-1875,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30672619)
高校博士点科研基金资助项目(200800231060)
关键词
人参
西洋参
栽培土壤
微生物种群
遗传多样性
RAPD
Panax ginseng C. A. Meyer
Panax quinquefolium L.
cultivating soil
microbial population
genetic diversity
RAPD
