摘要
采用RAPD-PCR分子标记技术分析了51株不同地理来源、寄主来源的绿僵菌Metarhizium anisopliae菌株的遗传多态性。从94条RAPD引物中筛选出18条引物,对所有试验菌株进行RAPD-PCR扩增,共获得96条扩增片段,其中81条片段表现多态性,占84.1%。聚类分析表明,供试的51株菌株间的相似性系数范围为0.52~0.98,表明菌株间存在丰富的遗传多态性。供试菌株在相似性系数0.7的水平可分为4个组群。按菌株DNA多态性与地理及寄主来源的聚类分析表明,大多数菌株的DNA多态性与地理或寄主有一定的相关性,即长期的地理环境和寄主适应性可能形成了种群的分化。
DNA polymorphism of 51 Metarhizium anisopliae isolates from different insect hosts and geographic origin was analyzed by random amplified polymorphic DNA（RAPD） technology.Eighteen primers were selected from 94 examined RAPD primers,which produced clear fingerprints by PCR amplification.In total,96 DNA fragments were amplified and 81 of which were polymorphic,reaching a percentage of 84.1%.Clustering analysis showed that the genetic similarity coefficients among different strains ranged from 0.52 to 0.98,suggesting that there is abundant DNA polymorphism among the isolates.The examined isolates were clustered into 4 groups at a similarity coefficient level of 0.7.Comparison of the origins of strains within and between groups indicates that much of the DNA polymorphism is associated with either geographical origin or insect host species.
出处
《中国生物防治》
CSCD
北大核心
2010年第4期428-435,共8页
Chinese Journal of Biological Control
基金
国家科技支撑计划项目(2006BAD08A02)
农业部948项目[2006-G54(2)]
国家高技术研究发展计划863项目(2006AA10A212)
关键词
绿僵菌
RAPD
遗传多态性
分子标记
种群分化
Metarhizium anisopliae
RAPD
polymorphism
molecular marker
population divergence
