摘要
目的 观察桑叶黄酮对胰岛素抵抗HepG2细胞胰岛素受体底物（IRS）蛋白表达的影响,探讨桑叶黄酮时胰岛素敏感性的作用及可能的分子机制.方法 用高胰岛素诱导培养HepG2细胞,建立胰岛素抵抗细胞模型,于培养液中加入桑叶黄酮共同孵育,观察桑叶黄酮对HepG2细胞模型葡萄糖掺入率的影响;应用Western blot等方法观察桑叶黄酮对模型细胞IRS蛋白表达的影响.结果 桑叶黄酮（0.01g/L）可增加胰岛素抵抗HepG2细胞的葡萄糖掺入率[（33.9±1.0）nmol/（mg·h）],与模型组[（27.9±1.1）nmol/（mg·h）]相比,差异有统计学意义（P＜0.01）;桑叶黄酮可使模型细胞IRS蛋白的表达显著增加（0.221±0.021）,与模型组（0.132±0.014）相比,差异有统计学意义（P＜0.01）.结论 桑叶黄酮能提高胰岛素的敏感性,改善胰岛素抵抗,其胰岛素增敏作用可能与胰岛素信号转导分子IRS蛋白的表达增强有关.
Objective To observe the role of mulberry flavone on the expression of insulin receptor substrate（IRS）in HepG2 cells with insulin resistance, and to explore the possible molecular mechanism that mulberry flavone improves insulin resistance. Methods HepG2 cells were cultured to establish insulin-resistance cell model in high concentration of insulin, and then incubated with mulberry flavone. Effect of mulberry flavone on the HepG2 cell model of glucose incorporation rate was observed. Western blot was used to observe the variety of IRS protein expression. Results Mulberry flavone increased the glucose incorporation rate of （33. 9 ± 1.0）higher than that of Conclusions Mulberry flavone can improve insulin resistance. Furthermore, IRS protein expression increasing is the possible molecular mechanism that mulberry flavone improves insulin resistance.
出处
《中国综合临床》
2010年第11期1129-1131,共3页
Clinical Medicine of China
基金
四川省重点科技项目（2006209-046）
四川省中医药管理局重点科技项目（200642）
