摘要
目的观察经皮自体骨髓移植在骨缺损瘢痕组织内的成骨作用，探索治疗骨不连的新途径。方法选健康家兔１８只，建立双侧桡骨中段骨及骨膜缺损１ｃｍ模型，６周后，实验侧（右）骨缺损区经皮注射自体红骨髓２ｍｌ，对照侧（左）骨缺损区经皮注射自身外周血２ｍｌ。在不同时间内进行Ｘ线片、组织学检查及新生组织钙、磷含量测定。临床应用经皮自体骨髓移植治疗骨不连１５例，其中腕舟骨不连７例，肱骨不连３例，股骨不连２例，胫骨不连３例。骨不连时间为７～４８个月，平均１３个月。结果实验侧骨缺损区在骨髓注射后不同时间的Ｘ线片、组织学检查均显示新骨形成逐渐增多，钙、磷含量均高于对照侧。对照侧无成骨现象。临床应用的１５例中，１３例在５～９个月骨折愈合，２例未愈合。结论经皮自体骨髓移植在骨缺损瘢痕组织内有成骨作用，在临床上可作为治疗骨不连的方法，尤其适用于骨不连部位软组织条件差、不具备开放植骨的病例。
Objective To observe the osteogenesis of percutaneous autogenous bone marrow grafting in cicatricial bone defect,to seek a good method for treating fracture nonunion.Methods Eighteen rabbits were adopted in this study.1 cm bone defect model was made in each side of radius, 6 weeks later,2 ml autogenous bone marrow was injected in the right radial bone defect as experimental group,2 ml autogenous peripheral blood in the left side as control group. X ray features,histologic changes, Ca and P content in the site of bone defect were studied in various times.Also 15 patients were treated clinically for the nonunion fracture,the average time from nonunion to bone marrow grafting was 13 months.Results In experimental group, the increasing new bone tissue were observed in X ray and histologic examination.While in control group, no osteogenesis was observed.Ca and P content of experimental group was higher than that of control group.For the 15 patients ,13 cases healed in 5～9 months,2 cases failed.Conclusion Percutaneous autogenous bone marrow grafting is capable of osteogenesis in the cicatricial bone defects.It can be used in nonunion cases which are not fit for operation of bone grafting because of poor condition of the skin.
出处
《中国修复重建外科杂志》
CAS
CSCD
1999年第3期148-151,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
骨不连
骨髓移植
成骨作用
骨缺损
瘢痕
Fracture nonunion
Bone marrow grafting Percutaneous Osteogenesis
