摘要
从赤子爱胜蚓（Ｅｉｓｅｎｉａｆｏｅｌｉａｄ）粗制品中纯化出五种纤溶酶组分（Ⅰ、Ⅶ、Ⅷ、Ⅸ、Ⅹ），在ＰＡＧＥ中均呈现单一带，并富含酸性氨基酸，酪氨酸含量也较高；ＳＤＳ－ＰＡＧＥ测得分子量分别为３４０００、３０５００、２７０００、２４５００和２１５００；ｐＩ为ｐＨ３．８０～４．２５不等；最大吸收峰均在２７６ｎｍ；在ｐＨ６．０～１０．０及５０℃以下有较高的稳定性；最适ｐＨ在８．５左右；最适温度为（６０±２）℃。组分Ⅰ在７０℃（１０ｍｉｎ）时活性不变，对某些变性剂表现出一定耐受性，其活性不被贵州小慈菇蛋白酶抑制。
Five fibrinolytic enzymes (Ⅰ、Ⅶ、Ⅷ、Ⅸ、Ⅹ) were isolated and purified from the earthworm (Eisenia foelied).They were single band determined by PAGE.EFE components all contain a very high proportion of acidic amino acid (Asp and Glu) and rich in tyrosine. Their molecular weight is 34 000、30 500、27 000、24 500 and 21 500 determined by SDS PAGE. The isoelectric points is between pH 3.80 and 4.25. The maximum UV peak of fibrinolytic enzymes of earthworm (EFE) is at 276 nm. They are relatively stable in the pH range of pH 6.0～10.0, below 50℃. The activity of EFE I is constant at 70℃, 10 min. EFE I has more resistance to some dena turants and is not inhibited by protease inhibitor from minor arroahead from Guizhou. The optimal pH of five EFEs is about pH 8.5 and optimal temperature is (60±2)℃.
出处
《华西药学杂志》
CAS
CSCD
北大核心
1999年第2期98-101,共4页
West China Journal of Pharmaceutical Sciences
关键词
纯化
纤溶酶
组分
理化性质
赤子爱胜蚓
分子量
Purificatory fibrinolytic enzymes Physical and chemical property Amino acid composition Isoelectric point Molecular weight Stability
