摘要
[目的]探讨防御素(Human defensins,HNP1-3)和白细胞介素8(IL-8)在大肠湿热型溃疡性结肠炎(UC)结肠组织中的表达及两者之间的相关性,观察中药溃结灵煎剂对HNP1-3和IL-8的影响,探讨溃结灵治疗UC的作用机制。[方法]60例活动期UC(大肠湿热型)患者随机分为治疗组[溃结灵加柳氮磺胺吡啶(SASP)]、对照组各30例,采用常规免疫组化SP法检测2组治疗前后肠黏膜组织内HNP1-3及IL-8的蛋白表达情况。[结果]UC患者随着病变分级程度的增高,HNP1-3、IL-8细胞因子的表达逐渐增强。两者呈明显的正相关。2组治疗后自身比较HNP1-3、IL-8表达较治疗前明显减少(P<0.01,<0.05)。2组治疗后HNP1-3、IL-8差值比较差异有统计学意义(P<0.05)。[结论]溃结灵配合SASP治疗UC很可能是通过抑制IL-8等细胞因子对中性粒细胞的激活,不能或减少释放细胞毒性HNP1-3,使损伤的内皮细胞得以修复,继而达到治疗的目的。
[Objective]To study the correlation between Human defensins（HNP1-3）and IL-8 in ulcerative colitis（UC）of large intestine damp-heat type and to observe the effect of Kuijie ling（KL）in treating UC.[Methods]Sixty patients of active UC（damp-heat syndrome）were randomly divided into 2groups,treatment group（KL and SASP）and control group.Protein expression of HNP1-3and IL-8in all cases were detected by immunochemistry SP method.[Results]The expressions of HNP1-3and IL-8in colon mucosa of UC patients tended to be more as the pathological grade was higher.The expression of HNP1-3was significantly correlated with that of IL-8.The expressions of HNP1-3and IL-8were decreased by KL and SASP treatment（P0.05）.There were outstanding differences in HNP1-3and IL-8expressions between the treatment group and the control group（P0.05）.[Conclusion]KL and SASP could treat UC probably by suppressing the activation of IL-8on neutrocytes,thus reducing HNP1-3release,resulting in the restore of the injured endothelial cells.
出处
《中国中西医结合消化杂志》
CAS
2010年第5期294-296,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
广东省中医药局建设中医药强省科研课题(2009044)
