摘要
目的:了解鼠中自然感染伯氏疏螺旋体的状况。方法:用巢式PCR扩增鼠中的伯氏疏螺旋体5S-23srRNA间隔区片段,对阳性产物进行测序。结果:在安吉县捕到鼠109只。分别在黑线姬鼠、青毛鼠中检到阳性6份,阳性率分别为5%和13%。结论:初步认为安吉县存在伯氏疏螺旋体病原。可能黑线姬鼠、青毛鼠为伯氏疏螺旋体宿主,尚需进一步调查。
Objective：To investigate the natural infection of Borrelia burgdorferi sensu lato in rodents from Zhejiang province.Methods： A Nest-PCR based on 5S-23srRNA intergenic spacer was developed and applied to detect Borrelia burgdorferi sensu lato in rodents.The positive PCR products were sequenced and analyzed.Results： Out of 109 rodents captured in Anji City in Zhejiang,6 rodents were positive for Borrelia burgdorferi sensu lato.The positive rates in rice field rat,Berlmys bowers and Apdemus agraius were 5% and 13%.Conclusion ：It suggested that there was Borrelia burgdorferi sensu lato existed in Zhejiang Province.Rice field mouse,Apodemus and shrew are possible hosts of Borrelia But further investigation is still needed.
出处
《中国卫生检验杂志》
CAS
2010年第11期2844-2845,共2页
Chinese Journal of Health Laboratory Technology
关键词
鼠类
伯氏疏螺旋体
聚合酶反应
Rodents
Borrelia burgdorferi sensu lato
Polymerase chain reaction（PCR）
