摘要
通过对内蒙古呼和浩特地区分离3株奶牛乳腺炎大肠杆菌16S-23S rRNA间隔区的PCR检测、基因测序和序列比较分析,结果表明:分离菌株12C与参考菌株的同源率为99.9%,DG-25A和DG-23A与参考菌株的同源率为98.9%,3株分离菌株的同源性高。应用PCR检测奶牛乳腺炎大肠杆菌的方法简便、快速、特异,该方法也可广泛用于乳房炎其他病原菌的检测。
Three isolates obtained from Huhhot region in inner Mongolia were investigated by PCR, sequences and analyses w/th oligonucleotide primers designed according to species - specific parts of the 16S - 23S rRNA spacer region of the species. The results showed that the sequence homology was 99.9% between isolate 12C and reference strain and it was 98.9% between strain DG25A, DG23A and reference strain. There was higher homology within three isolates. The PCR amplification of species - specific sequences of Escherichia call in the study offers a rapid, specific and sensitivity method to diagnose the mastitis.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2006年第4期58-61,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
