摘要
目的建立更简单、易行的组织工程气管上皮细胞与成纤维细胞培养方法。方法同时分离气管上皮细胞与成纤维细胞,共同培养,根据两种细胞对胰酶浓度耐受性不同的特性,分离气管上皮细胞与成纤维细胞,通过细胞生长曲线与MTT检测分析观察细胞的生长状态,并与常规方法培养的细胞进行比较。结果共同培养的两种细胞生长活性良好,用不同浓度胰酶消化后可完全分离两种细胞,分离后的细胞与常规方法培养的细胞生长曲线一致,MTT检测细胞增殖活性差异无统计学意义。结论该方法较常规方法简单、易行,是一种较好的体外一次获得两种细胞的培养方法。
Objective To explore an easier method to get epithelial cell and fibroblast from a rabbit＇s trachea for trachea tissue engineering in vitro. Methods Digest the epithehal ceil and fibroblast by trypsin after co-culture for 6 days. Distinguish these two kinds of ceils according to their different tolerances to trypsin and culture characteristics. The new method and the traditional method were compared by the proliferation of the ceils with ceil counting and MTT assay. Results The purity of the cells got with this new method is up to 100%. The ceils proliferation is as same as that cultured by traditional methods. Conclusion With this method, one can get two kinds of different ceils from the original in one time, it is easier to practice than the traditional.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2005年第6期362-364,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
基金
本课题受国家自然科学基金(39970707)陕西省自然科学基金(99SM37)资助
