摘要
目的:筛查卵巢癌组织中差异蛋白,探讨这些蛋白与卵巢癌发病之间的关系。方法:收集临床正常卵巢组织标本12例作为对照组,卵巢癌组织标本19例作为实验组,提取组织蛋白,采用Pathway Array技术,用37种磷酸化抗体、73种非磷酸化抗体分析卵巢癌和正常卵巢组织中蛋白表达的差异,利用QUANTITY ONE分析软件分析图片结果计算蛋白表达量,并对结果进行统计学分析。结果:共52种蛋白有表达(47%),其中23种(21%)蛋白表达差异显著(P<0.05)。与正常卵巢组织比较,卵巢癌组织p-Stat3、p-PDK1、p-cdc2、p-p38、p-AKT、XIAP、PCNA、cdc2p34、p53、Cdk2、Mesothelin、E-cadherin蛋白表达水平明显升高(P<0.05);而ERK、Cdc25C、Wee1、Akt、N-cadherin、α-tubulin、Vimentin、cdc42、Bax、PTEN蛋白表达水平明显降低(P<0.05)。结合体外实验,与正常卵巢组织和正常卵巢细胞系比较,卵巢癌组织和卵巢癌细胞系中p-Stat3、p-PKD1和p-AKT表达水平明显升高(P<0.05)。结论:Stat3、PKD1和AKT作为重要信号传导分子参与了卵巢癌的发生、发展。
Objective To detect the different proteins in ovarian carcinoma tissues and explore the relationship between the proteins and the carcinogenesis and development of ovarian carcinoma.Methods 19cases of serous carcinoma tissues and 12cases of normal ovarian tissues were collected,the proteins from tissues were extracted. With protein pathway array technique,37kinds of phosphorylative antibodies and 73kinds of non-phosphorylative antibodies,the different proteins in ovarian carcinoma tissues and normal ovarian tissues were detected.The results were analyzed by QUANTITY One software and statistically calculated.Results There were 52proteins expressed （47%）and 23proteins（21%）were diferentially expressed with statistical significance（P〈0.05）,including the over expression proteins：p-Stat3,p-PDK1,p-cdc2,p-p38,p-AKT,XIAP,PCNA,cdc2p34,p53,Cdk2,Mesothelin,E-cadherin and down-regulated proteins：ERK,Cdc25C,Wee 1,Akt,N-cadherin,α-tubulin,Vimentin,cdc42,Bax,PTEN （P〈0.05）.Compared with normal ovarian tissues and normal ovarian cell line,p-Stat3,p-PKD1and p-AKT were over expressed in ovarian carcinoma tissues and cell lines （P〈0.05）. Conclusion Stat3,PKD1and AKT play important roles in carcinogenesis of ovarian carcinoma
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期912-917,共6页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200705373)
